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Article
Reducing background absorbance via a double-lock strategy for detection of alkaline phosphatase and α-fetoprotein
Lowering the background signal for more sensitive analysis of determinands is as important as amplifying the target signal. The photoinduced oxidase of fluorescein has been reported, which can catalyze the oxi...
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Article
Bifunctional antibody and copper-based metal-organic framework nanocomposites for colorimetric α-fetoprotein sensing
Cu2+ are found to greatly reduce the photoinduced oxidase activity of fluorescein and then inhibit the chromogenic reaction catalyzed by fluorescein. A simple colorimetric assay for Cu2+ is established. Based on ...
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Article
One-pot synthesis of a composite consisting of the enzyme ficin and a zinc(II)-2-methylimidazole metal organic framework with enhanced peroxidase activity for colorimetric detection for glucose
An enzyme-metal organic framework (MOF) composite with saucer-like structure was prepared via one-pot synthesis from ficin (a cysteine proteolytic enzyme with POx activity), zinc(II) ions and 2-methylimidazole...
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Article
Hollow carbon dots labeled with FITC or TRITC for use in fluorescent cellular imaging
Hollow carbon dots (HCDs) were prepared by a solvothermal method and conjugated to either tetramethyl rhodamine isothiocyanate (TRITC) or fluorescein-5-isothiocyanate (FITC). This resulted in HCDs with bright ...
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Article
Open AccessIntrinsic Peroxidase-like Activity of Ficin
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we...
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Article
Maintenance of the activity of mono-dispersed Au and Ag nano-particles embedded in agar gel for ion-sensing and antimicrobial applications
Composite materials were synthesized by encapsulating Au and Ag nanoparticles in an agar gel matrix. These metallic nano-particles were found to be separately stored, so their optical, catalytic, and antibacte...
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Article
Gold Nanoparticles as a Probe for Prion Determination via Resonance Light Scattering Method
Dihydrolipoic acid (DHLA) modified gold nanoparticles (AuNPs) were used as a highly selective probe for the detection of prion proteins. We discovered that AuNPs undergo aggregation selectively in the presence...
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Article
A Carbon Dots-based Fluorescence Turn-on Method for DNA Determination
In the present work, we found that methylene blue (MB) caused an efficient quenching of the fluorescence of carbon dots (CDots). However, as ct-DNA was added, the fluorescence of CDots was restored reversibly....
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Article
Rapid Determination of Nanotoxicity Using Luminous Bacteria
A new luminescence-based toxicity test using luminous bacteria as a reporting agent has been developed to determine EC50 of different nanomaterials, such as gold nanoparticles and carbon nanotubes on living organ...