-
Article
Open AccessFirst direct evidence for direct cell-membrane penetrations of polycationic homopoly(amino acid)s produced by bacteria
Bacteria produce polycationic homopoly(amino acid)s, which are characterized by isopeptide backbones. Although the biological significance of polycationic homopoly(amino acid)s remains unclear, increasing atte...
-
Article
Open AccessGeneration of Fusarium oxysporum-suppressive soil with non-soil carriers using a multiple-parallel-mineralization technique
Disease-suppressive soils exist worldwide. However, the disease-suppression mechanism is unknown, and it’s unclear how to produce such soils. The microbiota that develop in a multiple-parallel-mineralization s...
-
Article
Activation of Cryptic Secondary Metabolite Biosynthesis in Bamboo Suspension Cells by a Histone Deacetylase Inhibitor
Plants have evolved a diverse array of secondary metabolite biosynthetic pathways. Undifferentiated plant cells, however, tend to biosynthesize secondary metabolites to a lesser extent and sometimes not at all...
-
Article
Bioproduction of 4-Vinylphenol and 4-Vinylguaiacol β-Primeverosides Using Transformed Bamboo Cells Expressing Bacterial Phenolic Acid Decarboxylase
Phenolic acid decarboxylase (PAD) catalyzes the decarboxylation of hydroxycinnamic acids to produce hydroxystyrenes, which serve as starting materials for the production of polymers. Bamboo (Phyllostachys nigra; ...
-
Article
A rare case of Helicobacter pylori-uninfected intramucosal poorly differentiated adenocarcinoma that occurred in the gastric fornix
We report a rare case of undifferentiated-type intramucosal gastric cancer that occurred in the fornix of the stomach without Helicobacter pylori infection, which consisted mainly of poorly differentiated adenoca...
-
Article
One-Step Enzymatic Synthesis of 1-Tuliposide A Using Tuliposide-Converting Enzyme
6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. They serve as precursors of the antimicrobial α-methyle...
-
Article
Photoautotrophic cultivation of oleaginous microalgae and co-pelletization with filamentous fungi for cost-effective harvesting process and improved lipid yield
Oleaginous microalga Scenedesmus obliquus SIT06 was selected as potential biodiesel feedstocks due to its high lipid content and suitable fatty acid composition for production of biodiesel with high oxidative sta...
-
Article
Open AccessRational metabolic-flow switching for the production of exogenous secondary metabolites in bamboo suspension cells
The synthetic biology-driven production of high-value plant secondary metabolites in microbial hosts has attracted extensive attention despite various challenges, including correct protein expression and limit...
-
Article
Open AccessTranscriptional alterations during proliferation and lignification in Phyllostachys nigra cells
Highly-lignified culms of bamboo show distinctive anatomical and mechanical properties compared with the culms of other grass species. A cell culture system for Phyllostachys nigra has enabled investigating the a...
-
Chapter
Plant Cell, Tissue, and Organ Culture Approaches to Explore the Functional Cell Differentiation in Phyllostachys and Bambusa Bamboo Plants
We present two case studies developed in our laboratory that involve the establishment of efficient plant cell tissue and organ culture (PCTOC) models to explore functional differentiation of cells responsible...
-
Article
Erratum to: Two new β-glucosidases from ethanol-fermenting fungus Mucor circinelloides NBRC 4572: enzyme purification, functional characterization, and molecular cloning of the gene
-
Article
Two new β-glucosidases from ethanol-fermenting fungus Mucor circinelloides NBRC 4572: enzyme purification, functional characterization, and molecular cloning of the gene
Two β-glucosidases (BGLs 1 and 2) were purified to homogeneity from the extracellular enzyme preparations of the ethanol-fermenting Mucor circinelloides NBRC 4572 statically grown on rice straw. BGLs 1 and 2 are ...
-
Article
Open AccessA novel xylogenic suspension culture model for exploring lignification in Phyllostachys bamboo
Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. ‘Bright Yellow 2’) and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) ar...
-
Article
A stand-alone adenylation domain forms amide bonds in streptothricin biosynthesis
A study of three synthetases involved in streptothricin biosynthesis demonstrates roles for two A domains in activating lysine, with one A domain transferring lysine to a carrier T domain and the second direct...
-
Article
A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress
A shoot multiplication system derived from internode explants was investigated with the aim of improving genetic characteristics of watercress (Nasturtium officinale R. Br.). Internodes of ca. 1 cm excised from i...
-
Article
Phenotypic alteration of interstitial cells of Cajal in idiopathic sigmoid megacolon
Interstitial cells of Cajal (ICCs) are detected as a pacemaker of gastrointestinal movement and express c-kit and CD34. Recently, ICCs have implicated pathogenesis in several human diseases presenting gastrointes...
-
Article
Molecular and enzymatic analysis of the “aldoxime–nitrile pathway” in the glutaronitrile degrader Pseudomonas sp. K-9
A gene cluster responsible for aldoxime metabolism in the glutaronitrile degrader Pseudomonas sp. K-9 was analyzed genetically and enzymatically. The cluster was composed of genes coding for aldoxime dehydratase ...
-
Article
Gigantic gastrointestinal stromal tumor in the pelvis
-
Article
Gastropleural fistula derived from malignant lymphoma
An 88-year-old man developed pneumothorax and pleural effusion. After the finding of ingesta in the pleural effusion, a gastropleural fistula was diagnosed. A biopsy specimen of the stomach revealed non-Hodgki...
-
Article
Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes
A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as Rhodococcus sp. The strain contained a novel ald...
