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  1. No Access

    Article

    Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans

    Genetic transformation of many filamentous fungi is carried out by a protocol that utilizes polyethylene glycol (PEG) and calcium ion (Ca2+). This method has remained practically unchanged for more than 20 years,...

    Tetsuya Kuwano, Chikako Shirataki, Yasuo Itoh in Current Genetics (2008)

  2. No Access

    Article

    Molecular Characterization of Japanese Sillago Vitellogenin and Changes in Its Expression Levels on Exposure to 17β-Estradiol and 4-tert-Octylphenol

    We cloned a full-length cDNA encoding vitellogenin (VTG) from a marine teleost, the Japanese sillago Sillago japonica. The cloned sillago VTG contained signal peptide, lipovitellin heavy chain, phosvitin, lipovit...

    Sung Ho Yoon, Yasuo Itoh, Gen Kaneko, Makiko Nakaniwa… in Marine Biotechnology (2008)

  3. No Access

    Article

    Suppression of tandem-multimer formation during genetic transformation of the mycotoxin-producing fungus Penicillium paxilli by disrupting an orthologue of Aspergillus nidulans uvsC

    An orthologue of Aspergillus nidulans uvsC and Saccharomyces cerevisiae RAD51 was cloned from the filamentous fungus, Penicillium paxilli. A mutation in uvsC causes UV sensitivity during germination. The product ...

    Mayumi Shibayama, Kazuhiro Ooi, Richard Johnson, Barry Scott… in Current Genetics (2002)

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    Article

    Paxilline-negative mutants of Penicillium paxilli generated by heterologous and homologous plasmid integration

    Using a monoclonal antibody based ELISA, 600 pAN7-1 plasmid-tagged mutants of Penicillium paxilli were screened for paxilline accumulation and one paxilline-negative mutant, YI-20, was identified. A molecular an...

    Carolyn Young, Yasuo Itoh, R. Johnson, I. Garthwaite… in Current Genetics (1998)

  5. No Access

    Article

    Hyphal anastomosis and complementary growth of fused cells inAlternaria alternata

    Hyphal anastomosis and complementary growth of fused cells inAlternaria alternata were investigated. Sixty-four experimental isolates were divided into anastomosis-positive (A+) and anastomosis-negative (A−) grou...

    Si-Liang Huang, Yasuo Itoh, Keisuke Kohmoto, Hiroshi Otani… in Mycoscience (1996)

  6. No Access

    Article

    Heterologous and homologous plasmid integration at a spore-pigment locus in Penicillium paxilli generates large deletions

    Mutations in a spore pigmentation locus (brs; brown spore) in Penicillium paxilli were isolated at a relatively-high frequency (0.17%) following integrative transformation of the hygromycin-resistance plasmid pAN...

    Yasuo Itoh, Barry Scott in Current Genetics (1994)

  7. No Access

    Article

    Integrative transformation of the mycotoxin-producing fungus, Penicillium paxilli

    A high frequency transformation system has been developed for Penicillium paxilli using pAN7-1. Up to 44% of the primary transformants were heterokaryons. Loss of hygromycin resistance was observed in primary tra...

    Yasuo Itoh, Richard Johnson, Barry Scott in Current Genetics (1994)