-
Article
Screening of TACE peptide inhibitors from phage display peptide library
To obtain the recombinant tumor necrosis factor-α converting enzyme (TACE) ectodomain and use it as a selective molecule for the screening of TACE peptide inhibitors, the cDNA coding catalytic domain (T800) an...
-
Article
Applied study on magnetic nanometer beads in preparation of genechip samples
A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobe...
-
Article
Mechanism of three inhibitors of TACE in blocking the converting of pro-TNFα into sTNFα
The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulatedin vitro with LPS intraven...
-
Article
Study of the effects of LPS on the TACE gene expression and its function
In order to investigate the effects of LPS on the TACE gene transcription and expression and its regulating effect on the TM-TNF secretion, in vitro studies were carried out on HL-60 cells stimulated by LPS. T...
-
Article
Relationship between the increase of secretion of sTNFα induced by lipopolysaccharides and the enhanced expression of TACE mRNA in HL-60 cells and adhesive cells from human spleen
In order to find out the potential modulators which influence the secretion of TNFα, the relationship between the amount of secreted TNFα(sTNFα) and the level of TNFα converting enzyme (TACE) gene expression w...
-
Article
Microcalorimetric study of the effect of porphyrin-cholesterol esters on cancer cells HL-60
By using an LKB-2277 Bioactivity Monitor, the effect of porphyrin-cholesterol esters on cancer cells HL-60 were examined at 25°C. There is a difference in the shape of the thermogenesis curve samong different ...
-
Article
Metabolic effect of odd—numbered medium chain fatty acids on glucose homeostasis and mobilization of depot fat in fasting rats
64 male rats were divided into two groups and fed on the diet containing, as percentage of dietary energy, 20% protein, 50% carbohydrate and 30% fat, which composed of undecanoin: caprin: soyabean oil (45:45:1...