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Article
Open AccessPublisher Correction: Semaphorin 3A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells
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Article
Open AccessSemaphorin 3A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells
Semaphorin-3A (SEMA3A) functions as a chemorepulsive signal during development and can affect T cells by altering their filamentous actin (F-actin) cytoskeleton. The exact extent of these effects on tumour-spe...
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Article
Bioengineering translational models of lymphoid tissues
Bioengineered models of human tissues have the potential to revolutionize human health by providing a translational platform to investigate disease mechanisms and develop new therapies. Recreating human immune...
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Protocol
Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering
Posttranscriptional silencing by microRNAs (miRNAs) is a critical constituent of eukaryotic gene regulation. miRNAs are short (~22 nt) noncoding RNAs capable of specifically targeting the miRNA-induced silenci...
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Article
Open AccessMulti-objective optimization reveals time- and dose-dependent inflammatory cytokine-mediated regulation of human stem cell derived T-cell development
The generation of T-cells from stem cells in vitro could provide an alternative source of cells for immunotherapies. T-cell development from hematopoietic stem and progenitor cells (HSPCs) is tightly regulated...
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Article
Open AccessAddendum: Precise tuning of gene expression levels in mammalian cells
Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions ...
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Article
Open AccessDecoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from ...
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Article
Open AccessPrecise tuning of gene expression levels in mammalian cells
Precise, analogue regulation of gene expression is critical for cellular function in mammals. In contrast, widely employed experimental and therapeutic approaches such as knock-in/out strategies are more suita...
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Article
Open AccessIn situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
RNA regulatory elements (RREs) are an important yet relatively under-explored facet of gene regulation. Deciphering the prevalence and functional impact of this post-transcriptional control layer requires tech...
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Protocol
Interrogation of Functional miRNA–Target Interactions by CRISPR/Cas9 Genome Engineering
Post-transcriptional silencing by microRNAs (miRNAs) is a critical constituent of eukaryotic gene regulation. miRNAs are short (~22nt) noncoding RNAs capable of specifically targeting the miRNA-induced-silenci...
