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Article
Antimicrobial Activities of Selected Cyathus Species
Twelve selected Cyathus species were tested for their abilities to produce antimicrobial metabolites. Most of them were found to produce secondary exo-metabolites that could induce morphological abnormalities of ...
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Article
Preliminary results on nematicidal activity from culture filtrates of Basidiomycetes against the pine wood nematode,Bursaphelenchus xylophilus (Aphelenchoididae)
One hundred and eighty one fungal species that were isolated from the fresh fruiting bodies collected in the Mountains of Pu Er County of Yunnan Province, China were tested on the pine wood nematode,Bursaphelench...
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Article
Nematicidal activity of Paecilomyces spp. and isolation of a novel active compound
Many species of Paecilomyces are entomogenous fungi and several are efficacious toward nematodes. To study the potential of Paecilomyces species in controlling nematodes, fungal extracts of 40 Paecilomyces spp. w...
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Article
Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system
Clostridium includes a number of species, such as thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum, producing biofuels and chemicals from lignocellulose, while gene...
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Article
Open AccessThe contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons
Clostridium thermocellum is a thermophilic anaerobic bacterium that degrades cellulose by using a highly effective cellulosome, a macromolecular complex consisting of multiple cellulose degrading enzymes organize...
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Article
Open AccessA novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum
Clostridium cellulolyticum and other cellulolytic Clostridium strains are natural producers of lignocellulosic biofuels and chemicals via the consolidated bioprocessing (CBP) route, and systems...
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Article
Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation
Cellulosomes are multi-enzyme complexes assembled by cellulases and hemicellulases through dockerin-cohesin interactions, which are the most efficient system for the degradation of lignocellulosic resources in...
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Article
Open AccessEfficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotei...
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Article
Open AccessDetermination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles...
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Article
Open AccessFirmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique + 1 transcriptional slippage
Bacterial insertion sequences (ISs) are ubiquitous mobile genetic elements that play important roles in genome plasticity, cell adaptability, and function evolution. ISs of various families and subgroups conta...
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Article
Open AccessConstruction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for ...
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Article
Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress
Butanol inhibits bacterial activity by destroying the cell membrane of Clostridium acetobutylicum strains and altering functionality. Butanol toxicity also results in destruction of the phosphoenolpyruvate-carboh...
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Article
Open AccessBiological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9
An anaerobic thermophilic bacterial strain, A9 (NITE P-03545), that secretes β-glucosidase was newly isolated from wastewater sediments by screening using esculin. The 16S rRNA gene sequence of strain A9 had 1...
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Article
Open AccessBioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils
Lignocellulose is a valuable carbon source for the production of biofuels and biochemicals, thus having the potential to substitute fossil resources. Consolidated bio-saccharification (CBS) is a whole-cell-bas...