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Article
Characterization and High Expression of a Novel Alkalinegh11 Xylanase in Pichia pastoris
A novel alkaline xylanase gene (xynAI) from Bacillus amyloliquefaciens was highly expressed in Pichia pastoris, and the enzyme was purified and characterized. Sequence analysis indicated that XynAI belongs to xyl...
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Article
Electronic Structures and Spectra Properties of Plasma-Embedded Atoms or Ions Under the External Electric-Field
A method within the relativistic computational scheme is developed and used to investigate the electronic structures and spectra properties of plasma-embedded atoms/ions placed in an external electric-field by...
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Article
A Novel Method for the Polarographic Determination of Trace Nitrite in Water
A method for polarographic determination of nitrites in water is developed. Nitrites firstly react with resorcinol, then the nitroso product reacts with nickel ion to form a complex, which can be reduced on dr...
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Article
Distinct properties of two glutamine synthetase isoforms in soybean root nodules
Glutamine synthetase (GS) in nodule cytosol plays a major role in the assimilation of the ammonium produced by biological nitrogen fixation. To characterize the GS protein family in Glycine max root nodules, the ...
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Article
Novel glyphosate-resistant aroA Gene from Paracoccus denitrificans in transgenic Arabidopsis
To date, only aroA variants derived from Agrobacterium tumefaciens CP4 have been used to generate the commercial glyphosate-resistant crops currently available in the market. Therefore, it is of interest to incre...
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Article
Experimental investigations and large-eddy simulation of low-swirl combustion in a lean premixed multi-nozzle combustor
This paper presents laser diagnostic experiments and large-eddy simulations (LES) of low-swirl lean premixed methane/air flames in a multi-nozzle combustor including five nozzles with the same structure. OH p...
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Article
Heterologous extracellular expression and initial characterization of the peroxisomal catalase from the methylotrophic yeast Hansenula polymorpha in Pichia pastoris
Catalase is well known to eliminate H2O2 in cells and reduces the toxicity of peroxide compounds. A catalase gene HpCAT1 of methylotrophic yeast Hansenula polymorpha without the part coding the native signal pept...
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Article
Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)
A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK c...
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Article
Wear properties of alloyed layers produced by laser surface alloying of pure titanium with B4C and T{i} mixed powders