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Article
Analysis of endophytic bacterial flora of mulberry cultivars susceptible and resistant to bacterial wilt using metagenomic sequencing and culture-dependent approach
Endophytes have a wide range of potential in maintaining plant health and sustainable agricultural environmental conditions. In this study, we analysed the diversity of endophytic bacteria in four mulberry cul...
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Article
Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria
To investigate the biochemical characterization of the carboxylesterase LmCesA1 from Locusta migratoria.
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Article
Curcusone C induces apoptosis in endometrial cancer cells via mitochondria-dependent apoptotic and ERK pathway
Jatropha curcashas been used in traditional medicine in Africa to treat cancer for thousands of years. This study aimed to examine the anti-endometrial cancer effect of Curcusone C, a naturally occurring rhamnofo...
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Article
Improving the thermostability of a GH97 dextran glucosidase by rational design
This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using ...
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Article
Improve uridine production by modifying related metabolic pathways in Bacillus subtilis
The metabolic pathway related to uridine production was modified in Bacillus subtilis in order to increase the production of uridine.
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Article
Improving RNA content of salt-tolerant Zygosaccharomyces rouxii by atmospheric and room temperature plasma (ARTP) mutagenesis and its application in soy sauce brewing
Derived from RNA, 5ʹ-ribonucleotides, especially Inosine-5ʹ-monophosphate (IMP) and guanosine-5ʹ-monophosphate (GMP), can enhance the umami taste of soy sauce. In this study, the RNA content of three different...
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Article
Efficient production of red Monascus pigments with single non-natural amine residue by in situ chemical modification
Filamentous fungi Monascus sp. has been utilized for fermentative production of food colorant (Red Yeast Rice) for more than 1000 years in China. The main colorant components of Red Yeast Rice are mixture of red
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Article
Enhanced biosynthesis of phenazine-1-carboxamide by Pseudomonas chlororaphis strains using statistical experimental designs
Phenazine-1-carboxamide (PCN) is one of the major biocontrol agents produced by plant growth-promoting rhizosphere (PGPR) pseudomonads including Pseudomonas chlororaphis. In this study, a combined strategy of gen...
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Article
Engineering Pseudomonas for phenazine biosynthesis, regulation, and biotechnological applications: a review
Pseudomonas strains are increasingly attracting considerable attention as a valuable bacterial host both for basic and applied research. It has been considered as a promising candidate to...
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Article
Cloning and transcription analysis of the nitrate reductase gene from Haematococcus pluvialis
To optimize the cultivation media for the growth rate of Haematococcus pluvialis and to study the transcription regulation of the algal nitrate reductase (NR), a key enzyme for nitrogen metabolism.
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Article
Dynamic changes of histone H3 lysine 9 following trimethylation in bovine oocytes and pre-implantation embryos
We have examined dynamic changes of histone H3 lysine 9 following trimethylation (H3K9me3), the mRNA expression levels of SUV39H1 and SUV39H2 in bovine oocytes and the role in the development of in vitro fertiliz...
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Article
A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor
To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source.
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Article
High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris
A novel, high-level expression, thermostable mannan endo-1,4-beta-mannosidase is urgently needed for industrial applications.
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Article
Sodium butyrate-induced histone hyperacetylation up-regulating WT1 expression in porcine kidney fibroblasts
Wilms’ tumor 1 gene (WT1) is essential for the development of kidney and histone acetylation and is involved in its expression regulation in mice. However, whether WT1 expression is associated with histone acetyl...
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Article
An efficient alternative marker for specific identification of Mycobacterium tuberculosis
Rapid and accurate identification of mycobacteria to the species level is important to provide epidemiological information and to guide the appropriate treatment, especially identification of the Mycobacterium tu...
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Article
Production of lycopene by metabolically-engineered Escherichia coli
Escherichia coli strain CAR001 that produces β-carotene was genetically engineered to produce lycopene by deleting genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene β-cyclase (crt...
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Article
Open AccessHeterologous expression and characterization of a malathion-hydrolyzing carboxylesterase from a thermophilic bacterium, Alicyclobacillus tengchongensis
A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecul...
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Article
Evaluation of probiotic properties of Lactobacillus plantarum strains isolated from Chinese sauerkraut
Lactobacillus plantarum strains isolated and identified from naturally-fermented Chinese sauerkraut were examined in vitro for potential probiotic properties and in vivo for cholesterol-lowering ...
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Article
Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model
The probiotic adjunct Lactobacillus plantarum K25 was inoculated into milk to produce probiotic cheese. The effect of Lb. plantarum K25 on cheese composition, microbiological growth and survival during the manufa...
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Article
Metabolic engineering of Bacillus subtilis for enhanced production of acetoin
Acetoin is widely used in food and other industries. A bdhA and acoA double-knockout strain of Bacillus subtilis produced acetoin at 0.72 mol/mol, a 16.4 % increased compared to the wild type. Subsequent overexpr...