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Article
Open AccessDevelopment of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis
Zymomonas mobilis is an important industrial bacterium ideal for biorefinery and synthetic biology studies. High-throughput CRISPR-based genome editing technologies have been developed to enable targeted engineer...
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Article
Open AccessEstablishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis
Efficient and convenient genome-editing toolkits can expedite genomic research and strain improvement for desirable phenotypes. Zymomonas mobilis is a highly efficient ethanol-producing bacterium with a small gen...
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Article
Carbon Nanoparticles and Graphene Nanosheets as MALDI Matrices in Glycomics: a New Approach to Improve Glycan Profiling in Biological Samples
Glycomics continues to be a highly dynamic and interesting research area due to the need to comprehensively understand the biological attributes of glycosylation in many important biological functions such as ...
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Article
Open AccessThe artificial neural network approach based on uniform design to optimize the fed-batch fermentation condition: application to the production of iturin A
Iturin A is a potential lipopeptide antibiotic produced by Bacillus subtilis. Optimization of iturin A yield by adding various concentrations of asparagine (Asn), glutamic acid (Glu) and proline (Pro) during the ...