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Protocol
Detection of Glycoproteins in Gels and Blots
As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniq...
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Protocol
Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes such as Ponceau Red, Amido Black, Fast Green, or most commonly Coomassie Brilliant Blue (1,2). Alternatively, sensitive detection met...