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Article
Open AccessConformations of Bcs1L undergoing ATP hydrolysis suggest a concerted translocation mechanism for folded iron-sulfur protein substrate
The human AAA-ATPase Bcs1L translocates the fully assembled Rieske iron-sulfur protein (ISP) precursor across the mitochondrial inner membrane, enabling respiratory Complex III assembly. Exactly how the folded...
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Article
Open AccessSequence-structure-function characterization of the emerging tetracycline destructase family of antibiotic resistance enzymes
Tetracycline destructases (TDases) are flavin monooxygenases which can confer resistance to all generations of tetracycline antibiotics. The recent increase in the number and diversity of reported TDase sequen...
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Article
Open AccessA human antibody epitope map of the malaria vaccine antigen Pfs25
Pfs25 is a leading antigen for a malaria transmission-blocking vaccine and shows moderate transmission-blocking activity and induction of rapidly decreasing antibody titers in clinical trials. A comprehensive ...
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Article
Open AccessStructural analysis of Plasmodium falciparum ookinete surface antigen Pfs28 relevant for malaria vaccine design
Pfs28 is a Plasmodium falciparum malaria transmission-blocking vaccine candidate that is anchored to the parasite surface through a C-terminal glycosylphosphatidylinositol (GPI) moiety, and plays a role in parasi...
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Article
Open AccessNeutralizing and interfering human antibodies define the structural and mechanistic basis for antigenic diversion
Defining mechanisms of pathogen immune evasion and neutralization are critical to develop potent vaccines and therapies. Merozoite Surface Protein 1 (MSP-1) is a malaria vaccine antigen and antibodies to MSP-1...
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Article
Open AccessAuthor Correction: A human monoclonal antibody blocks malaria transmission and defines a highly conserved neutralizing epitope on gametes
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Article
Open AccessA human monoclonal antibody blocks malaria transmission and defines a highly conserved neutralizing epitope on gametes
Malaria elimination requires tools that interrupt parasite transmission. Here, we characterize B cell receptor responses among Malian adults vaccinated against the first domain of the cysteine-rich 230 kDa gam...
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Article
Open AccessCrystal structure of P. falciparum Cpn60 bound to ATP reveals an open dynamic conformation before substrate binding
Plasmodium falciparum harbors group 1 and group 2 chaperonin systems to mediate the folding of cellular proteins in different cellular locations. Two distinct group 1 chaperonins operate in the organelles of mito...
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Article
Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein
The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron–sulfur protein (ISP), a subunit of...
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Article
Open AccessStructural basis for nucleotide-modulated p97 association with the ER membrane
Association of the cytosolic AAA (ATPases associated with various cellular activities) protein p97 to membranes is essential for various cellular processes including endoplasmic reticulum (ER)-associated degra...
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Article
Open AccessRole of the D1-D2 Linker of Human VCP/p97 in the Asymmetry and ATPase Activity of the D1-domain
Human AAA+ protein p97 consists of an N-domain and two tandem ATPase domains D1 and D2, which are connected by the N-D1 and the D1-D2 linkers. Inclusion of the D1-D2 linker, a 22-amino acid peptide, at the end of...
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Article
Cloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region
Antiquitin (ALDH7) is a member of the aldehyde dehydrogenase superfamily. In plants, ALDH7 is inducible upon dehydration and is thus believed to possess an osmoregulatory role. On the other hand, however, its ...
