Skip to main content

43 Result(s)

within W.-D. Deckwer Remove this filter

previous disabled Page of 3
and

  1. No Access

    Chapter and Conference Paper

    Use of Hydrocyclone as an Efficient Tool for Cell Retention in Perfusion Cultures

    A new specially designed hydrocyclone was successfully applied as cell separation system in 6-L and 30-L continuously perfused bioreactors. Cell concentration reached up to 10 million per mL and viability rema...

    E.A. Elsayed, G.-W. Piehl, J. Nothnagel… in Animal Cell Technology Meets Genomics (2005)

  2. No Access

    Chapter

    Induction Kinetics of Apoptosis in Mammalian Cell Cultures

    The morphological and biochemical features of cell death in three production cell lines and one human carcinoma cell line were characterised in order to investigate the kinetics of apoptosis induction, apoptot...

    M. Linz, A.-P. Zeng, W.-D. Deckwer in Animal Cell Technology: Products from Cell… (2002)

  3. No Access

    Article

    Bacterial alginate: physiology, product quality and process aspects

    Alginate, a copolymer of β-D-mannuronic acid and α-L-guluronic acid and currently commercially produced from the marine brown algae, can also be biologically produced by bacteria such as Azotobacter vinelandii,

    W. Sabra, A.-P. Zeng, W.-D. Deckwer in Applied Microbiology and Biotechnology (2001)

  4. No Access

    Chapter

    The Use of Hydrocyclones for Mammalian Cell Retention in Perfusion Bioreactors

    A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%. Due to the fact that a 5-L-bioreactor was us...

    A. Jockwer, Prof Ricardo A. Medronho… in Animal Cell Technology: From Target to Mar… (2001)

  5. No Access

    Chapter

    Separation of Mammalian Cells Using a Specially Designed Rotating Disc Filter

    Through continuous fresh nutrient supply and metabolite removal, combined with cell retendon in the bioreactor, perfusion cultures allow high cell concentrations and product titres to be achieved at relatively...

    Ms Leda R. Castilho, F. Birger Anspach… in Animal Cell Technology: From Target to Mar… (2001)

  6. No Access

    Article

    Effect of phosphate and oxygen concentrations on alginate production and stoichiometry of metabolism of Azotobacter vinelandii under microaerobic conditions

    Alginate production by Azotobacter vinelandii was studied in batch and continuous cultures under microaerobic conditions. In batch culture at a pO2 of 2–3% (air saturation) alginate production was enhanced by de...

    W. Sabra, A. -P. Zeng, S. Sabry, S. Omar… in Applied Microbiology and Biotechnology (1999)

  7. No Access

    Article

    Microbial production of 1,3-propanediol

    1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out...

    H. Biebl, K. Menzel, A.-P. Zeng, W.-D. Deckwer in Applied Microbiology and Biotechnology (1999)

  8. No Access

    Article

    Cultivation of Tetrahymena thermophila in a 1.5-m3 airlift bioreactor

    A large-scale cultivation system for the mass cell production and extraction of the protozoon Tetrahymena thermophila has been developed on the basis of a low-cost complex nutrient medium. Cell growth and the pr...

    D. Hellenbroich, U. Valley, T. Ryll, R. Wagner… in Applied Microbiology and Biotechnology (1999)

  9. No Access

    Article

    Semisynthetic culture medium for growth and dihydroxyacetone production by Gluconobacter oxydans

    Only three vitamins (pantothenate, p-amino benzoic acid, nicotinic acid) and two amino acids (serine, glutamine) were required in the growth medium for Gluconobacter oxydans which allowed the concentration of ...

    M. Wethmar, W.-D. Deckwer in Biotechnology Techniques (1999)

  10. No Access

    Article

    Physiological characterization and cultivation strategies of the pentachlorophenol-degrading bacteria Sphingomonas chlorophenolica RA2 and Mycobacterium chlorophenolicum PCP-1

    The physiological characteristics of growth and pentachlorophenol degradation of the bacteria Sphingomonas chlorophenolica RA2 and Mycobacterium chlorophenolicum PCP-1 were studied quantitatively in liquid cultur...

    C Wittmann, A-P Zeng, W-D Deckwer in Journal of Industrial Microbiology and Biotechnology (1998)

  11. No Access

    Article

    Fermentation of glycerol to 1,3-propanediol and 2,3-butanediol by Klebsiella pneumoniae

    Klebsiella pneumoniae was shown to convert glycerol to 1,3-propanediol, 2,3-butanediol and ethanol under conditions of uncontrolled pH. Formation of 2,3-butanediol starts with some hours' delay and is accompanie...

    H. Biebl, A.-P. Zeng, K. Menzel, W.-D. Deckwer in Applied Microbiology and Biotechnology (1998)

  12. No Access

    Article

    Production of 1,3-propanediol by Clostridium butyricum in continuous culture with cell recycling

    The continuous fermentation of 1,3-propanediol from glycerol by Clostridium butyricum was subjected to cell recycling by filtration using hollow-fibre modules made from polysulphone. The performance of the cultu...

    A. Reimann, H. Biebl, W.-D. Deckwer in Applied Microbiology and Biotechnology (1998)

  13. No Access

    Article

    Influence of iron, phosphate and methyl viologen on glycerol fermentation of Clostridium butyricum

    The effect of methyl viologen addition, and iron and phosphate limitation on product distribution during glycerol fermentation of Clostridium butyricum DSM 5431 was investigated in continuous culture. Special att...

    A. Reimann, H. Biebl, W. -D. Deckwer in Applied Microbiology and Biotechnology (1996)

  14. No Access

    Article

    Simultaneous production and decomposition of clavulanic acid during Streptomyces clavuligerus cultivations

    Clavulanic acid (CA) was produced by Streptomyces clavuligerus in medium containing glycerol and soy meal or soy meal extract. With regard to growth and CA productivity, the microorganism showed significant diffe...

    A. F. Mayer, W. -D. Deckwer in Applied Microbiology and Biotechnology (1996)

  15. No Access

    Article

    Evaluation of the biodegradability of copolyesters containing aromatic compounds by investigations of model oligomers

    Model oligo esters of terephthalic acid with 1,2-ethanediol, 1,3-propanediol, and 1,4-butanediol have been investigated with regard to their biodegradability in different biological environments. Well-characte...

    U. Witt, R.-J. Müller, W.-D. Deckwer in Journal of environmental polymer degradation (1996)

  16. No Access

    Article

    Growth inhibition by ammonia and use of a pH-controlled feeding strategy for the effective cultivation of Mycobacterium chlorophenolicum

    The inhibitory effect of ammonia on the growth of the polychlorinated xenobiotic-degrading bacterium Mycobacterium chlorophenolicum was examined. The strain is inhibited by both the ionized and nonionized forms o...

    C. Wittmann, A.-P. Zeng, W.-D. Deckwer in Applied Microbiology and Biotechnology (1995)

  17. No Access

    Article

    Cellulase-free xylanase by thermophilic fungi: a comparison of xylanase production by two Thermomyces lanuginosus strains

    Thermomyces lanuginosus strains RT9 and MH4 were studied to find favourable cultivation conditions and to compare their abilities to produce xylanolytic enzymes in three media on different substrates at 50° C or...

    M. M. Hoq, W.-D. Deckwer in Applied Microbiology and Biotechnology (1995)

  18. No Access

    Article

    Calculation of carbon balances for evaluation of the biodegradability of polymers

    Establishing carbon balances has been proven to be an applicable and powerful tool in testing biodegradability of polymers. In controlled degradation tests at a 4-L scale with the model polymer poly(β-hydroxybuty...

    S. Urstadt, J. Augusta, R. -J. Müller… in Journal of environmental polymer degradati… (1995)

  19. No Access

    Article

    Effects of substrate limitation on product distribution and H2/CO2 ratio inKlebsiella pneumoniae during anaerobic fermentation of glycerol

    Product formation during anaerobic degradation of glycerol byKlebsiella pneumoniae DSM 2026, under glycerol limitation and glycerol excess in continugius cultures, has been investigated. Major and minor products ...

    B. O. Solomon, A. -P. Zeng, H. Biebl… in Applied Microbiology and Biotechnology (1994)

  20. No Access

    Article

    Interpretation of the gas residence time distributions in large stirred tank reactors

    The behaviour of dispersed gas in large aerated stirred tank reactors is modelled by means of a Markov-process, which distinguishes between small recirculation bubbles with “stagnant gas” content, large rising...

    H. M. Rüffer, A. Pethö, K. Schügerl, A. Lübbert, A. Ross… in Bioprocess Engineering (1994)

previous disabled Page of 3