9 Result(s)
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Volodymyr M. Korkhov
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Article
Open AccessStructural basis of connexin-36 gap junction channel inhibition
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Article
Open AccessStructural basis of the Meinwald rearrangement catalysed by styrene oxide isomerase
Membrane-bound styrene oxide isomerase (SOI) catalyses the Meinwald rearrangement—a Lewis-acid-catalysed isomerization of an epoxide to a carbonyl compound—and has been used in single and cascade reactions. Ho...
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Protocol
Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Characterization by Cryo-Electron Microscopy
Connexins are polytopic domain membrane proteins that form hexameric hemichannels (HCs) which can assemble into gap junction channels (GJCs) at the interface of two neighboring cells. The HCs may be involved i...
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Article
Open AccessStructural basis of organic cation transporter-3 inhibition
Organic cation transporters (OCTs) facilitate the translocation of catecholamines, drugs and xenobiotics across the plasma membrane in various tissues throughout the human body. OCT3 plays a key role in low-af...
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Article
Open AccessStructural basis of adenylyl cyclase 9 activation
Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is autoinhibited by the AC9 C-term...
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Article
Open AccessStructural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion
Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasm...
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Protocol
Affinity Purification of Membrane Proteins
Biochemical, biophysical, and structural studies of membrane proteins rely on the availability of highly pure and monodisperse membrane protein samples. One of the most powerful methods for isolation of the me...
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Article
On the Efficiency of NHS Ester Cross-Linkers for Stabilizing Integral Membrane Protein Complexes
We have previously presented a straightforward approach based on high-mass matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) to study membrane proteins. In addition, the stoichiometry ...
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Article
Open AccessPeptide-based interactions with calnexin target misassembled GABA transporter-1 molecules into organized smooth endoplasmic reticulum compartments*
