16 Result(s)
within
Vignesh Muthusamy
Biotechnology
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Article
Unique genetic architecture of prolificacy in ‘Sikkim Primitive’ maize unraveled through whole-genome resequencing-based DNA polymorphism
‘Sikkim Primitive’ maize landrace, unique for prolificacy (7–9 ears per plant) possesses unique genomic architecture in branching and inflorescence-related gene(s), and locus Zm00001eb365210 encoding glycosyltran...
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Genetic Improvement of Specialty Corn for Nutritional Quality Traits
Specialty corn has emerged as a preferred choice as food, feed, and various industrial products due to its diverse usage. Among the various types, sweet corn, popcorn, waxy corn, high amylose corn, high oil co...
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Dissection of QTLs for Biotic Stress Resistance in Maize
Biotic stresses are major concerns for the world’s food security. These, commonly induced by diseases and/or insect pests, are one of the primary reasons for maize yield and quality losses. About 10% of the an...
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Development and validation of rapid and cost-effective protocol for estimation of amylose and amylopectin in maize kernels
Maize possesses wide variation in amylose and amylopectin which assumes significance as a part of both food-chain and different industrial applications. Estimation of amylose and amylopectin in maize kernels i...
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Maize Breeding
Maize has emerged as an important crop for food, feed and various applications. Utilization of hybrid technology has resulted in a quantum jump in grain production worldwide. However, ever-increasing populatio...
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Cyanobacterial inoculation as resource conserving options for improving the soil nutrient availability and growth of maize genotypes
Harnessing the benefits of plant–microbe interactions towards better nutrient mobilization and plant growth is an important challenge for agriculturists globally. In our investigation, the focus was towards an...
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Article
Expression analysis of β-carotene hydroxylase1 and opaque2 genes governing accumulation of provitamin-A, lysine and tryptophan during kernel development in biofortified sweet corn
Traditional sweet corn possesses low levels of provitamin-A (proA), lysine and tryptophan. Mutant version of β-carotene hydroxylase1 (crtRB1) gene affecting the accumulation of β-carotene (BC), β-cryptoxanthin (B...
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Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch
A modified SDS–Trizol method was optimized for isolation of total RNA from the stored maize seeds at regular interval of one month for 4 months. Use of SDS extraction buffer before the use of Trizol reduced th...
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Influence of T-, C- and S- cytoplasms on male sterility and their utilisation in baby corn hybrid breeding
Baby corn has become a popular dietary choice worldwide. Here, we evaluated the potential of different male sterility systems as a tool to save cost for manual detasseling in baby corn. 24 hybrids having ferti...
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Article
Priming maize seeds with cyanobacteria enhances seed vigour and plant growth in elite maize inbreds
Biofilm formation of a nitrogen-fixing cyanobacterium Anabaena torulosa with a beneficial fungus Trichoderma viride (An-Tr) was examined under laboratory conditions. A gradual enhancement in growth over A. torulo...
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Article
Development and validation of breeder-friendly gene-based markers for lpa1-1 and lpa2-1 genes conferring low phytic acid in maize kernel
Based on C (wild) to T (mutant) transition at amino acid position 1432 bp of lpa1-1 gene, two dominant markers each specific to wild type (LPA1) and mutant (lpa1-1) allele were developed and validated across seve...
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Article
Molecular characterization of teosinte branched1 gene governing branching architecture in cultivated maize and wild relatives
We sequenced the entire tb1 gene in six maize inbreds and its wild relatives (parviglumis, mexicana, perennis and luxurians) to characterize it at molecular level. Hopscotch and Tourist transposable elements were...
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Biofortification of Maize for Protein Quality and Provitamin-A Content
Protein and micronutrient deficiency have emerged as the major public health problems in under-developed as well as develo** countries. Of the several approaches used to alleviate malnutrition, biofortificat...
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Article
Molecular analysis of mutant granule-bound starch synthase-I (waxy1) gene in diverse waxy maize inbreds
Waxy corn is popular beacuse of its high amylopectin due to mutation in granule-bound starch synthase-I or Waxy1 (Wx1) gene. Here, we characterized the wx1 allele among 24 diverse waxy inbreds using gene-based ma...
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Molecular characterization of 5′ UTR of the lycopene epsilon cyclase (lcyE) gene among exotic and indigenous inbreds for its utilization in maize biofortification
Maize grains are the important source of food and energy, but possess very low proA (< 2.5 µg/g) compared to target level of 15 µg/g set by HarvestPlus to alleviate VAD. Favorable allele having variation in 5′...
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Microsatellite marker-based characterization of waxy maize inbreds for their utilization in hybrid breeding
Waxy corn possesses 95–100% amylopectin, compared to 70–75% in normal maize, owing to mutation in Wx gene encoding a granule-bound starch synthase I. Amylopectin is used as an ingredient in textile, adhesive and ...
