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Article
FISHing in the microwave: the easy way to preserve proteins. I. Colocalization of DNA probes and surface antigens in human leukocytes
Most of the techniques for simultaneous visualization of proteic cell components and DNA probes are difficult to carry out. We have developed an alternative protocol for simultaneous visualization of DNA probe...
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Chapter
Chromosome differentiation using nucleases: an overview
The era of the longitudinal differentiation of metaphase chromosomes (chromosome banding) dawned in 1968 when Caspersson and coworkers described the use of quinacrine mustard as a fluorescent compound which he...
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Article
5-Azacytidine produces differential undercondensation of alpha, beta and classical human satellite DNAs
Fluorescencein situ hybridization employing human alphoid, beta and classical satellite DNA probes was performed on 5-azacytidine treated and untreated chromosomes obtained from human lymphocytes. The individual ...
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Article
Electron microscopy and biochemical analysis of mouse metaphase chromosomes after digestion with restriction endonucleases
Electron microscopy (EM) of whole mounted mouse chromosomes, light microscopy (LM), and agarose gel electrophoresis of DNA were used to investigate the cytological effect on chromosomes of digestion with the r...
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Article
The R-banding pattern of the chinese hamster Don cell line
Chinese hamster cells (Don line) were treated in vivo with 5-BrdU and 332580Hoechst fluorochrome for obtaining the partial inhibition of condensation that causes the R-banding pattern. Untreated chromosomes were ...