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  1. No Access

    Article

    Vanillin Resistance Induced by BssS Overexpression in Escherichia coli

    Overexpression of the BssS gene, a biofilm formation regulator, in planktonic Escherichia coli cells has been shown to confer the vanillin-resistant phenotype Vanr to the bacteria. The MG1655PL-tac-bssS strain st...

    M. V. Ovsienko, E. N. Fedorova, V. G. Doroshenko in Applied Biochemistry and Microbiology (2018)

  2. No Access

    Article

    Comparative Analysis of Mono- and Bifunctional Chorismate Synthases in Escherichia coli Cells Capable and Incapable of Phenylalanine Production

    The activity of chorismate synthase, the terminal enzyme of the common aromatic pathway, is absolutely dependent on reduced flavin mononucleotide. The bifunctional chorismate synthase of Saccharomyces cerevisiae ...

    A. E. Slesareva, L. G. Kuhn, V. G. Doroshenko in Applied Biochemistry and Microbiology (2017)

  3. No Access

    Article

    Metabolic engineering of Escherichia coli for the production of phenylalanine and related compounds

    In this review, the metabolic engineering approaches including those used by the authors in creating phenylalanine producers based on Escherichia coli were systematized. Optimization of the amino acid biosynthesi...

    V. G. Doroshenko, V. A. Livshits, L. G. Airich… in Applied Biochemistry and Microbiology (2015)

  4. No Access

    Article

    The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants

    Strain MG1655+ hisG r hisL′-ΔΔ, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal m...

    V. G. Doroshenko, A. O. Lobanov, E. A. Fedorina in Applied Biochemistry and Microbiology (2013)

  5. No Access

    Article

    Gene yddG of Escherichia coli encoding the putative exporter of aromatic amino acids: Constitutive transcription and dependence of the expression on the cell growth rate

    Gene yddG of Escherichia coli encodes a protein of the inner membrane. Data obtained earlier demonstrated that under conditions of aromatic amino acids overproduction YddG promotes their export from E. coli cells...

    I. S. Tsyrenzhapova, V. G. Doroshenko, L. G. Airich… in Russian Journal of Genetics (2009)

  6. No Access

    Article

    2D [1H,13C] NMR study of carbon fluxes during glucose utilization by Escherichia coli MG1655

    Carbon fluxes through main pathways of glucose utilization in Escherichia coli cells-glycolysis, pentose phosphate pathway (PPP), and Enther-Doudoroff pathway (EDP)—were studied. Their ratios were analyzed in E. ...

    A. D. Kivero, E. V. Bocharov, V. G. Doroshenko… in Applied Biochemistry and Microbiology (2008)

  7. No Access

    Article

    Tuning the Expression Level of a Gene Located on a Bacterial Chromosome

    A new method of constructing a set of bacterial cell clones varying in the strength of a promoter upstream of the gene of interest was developed with the use of Escherichia coli MG1655 and lacZ as a reporter. The...

    J. I. Katashkina, A. Yu. Skorokhodova, D. V. Zimenkov, A. Yu. Gulevich… in Molecular Biology (2005)

  8. No Access

    Article

    A model for failure in the contact system of a semiconductor device on temperature cycling

    A physical model is proposed for the failure in a multilayer contact system for a semiconductor device on temperature cycling.

    L. V. Bereza, A. A. Dolgov, V. G. Doroshenko… in Journal of engineering physics (1983)