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  1. No Access

    Article

    Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns

    Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since p...

    J. Fíla, V. Čapková, J. Feciková, D. Honys in Biologia Plantarum (2011)

  2. No Access

    Article

    Impact of in vitro Cultivation Conditions on Stress Responses and on Changes in Thylakoid Membrane Proteins and Pigments of Tobacco during ex vitro Acclimation

    Four physiologically and phenotypically diversified tobacco (Nicotiana tabacum L. cv. Samsun) plantlet variants had been generated by cultivation on media either lacking or containing sucrose (0 and 3 %, m/v) und...

    P. Hofman, D. Haisel, J. Komenda, M. Vágner, I. Tichá, C. Schäfer… in Biologia Plantarum (2002)

  3. No Access

    Article

    Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation

    Plant phenotype stability during ex vitro growth, one of the main requirements of plant micropropagation, was tested on tobacco. Plants cultivated in vitro in the presence of 3 % sucrose under photon flux density...

    D. Haisel, P. Hofman, M. Vágner, H. Lipavská, I. Tichá, C. Schäfer… in Biologia Plantarum (2001)

  4. No Access

    Article

    Temporal Changes in the RNA Distribution between Polysomes and Postpolysomal Ribonucleoprotein Particles in Tobacco Male Gametophyte

    Growth of tobacco (Nicotiana tabacum L.) pollen tube is controlled by post-transcriptionally regulated protein synthesis. Stored mRNA was found to be present in the form of messenger ribonucleoprotein particles (...

    D. Honys, V. Čapková in Biologia Plantarum (2000)

  5. No Access

    Article

    The pollen-specific gene Ntp303 encodes a 69-kDa glycoprotein associated with the vegetative membranes and the cell wall

    The pollen-specific gene Ntp303 belongs to the class of late pollen specific genes. It is first transcribed directly after pollen mitosis. Biochemical properties, appearance and precise location of the NTP303 pro...

    F. R. A. Wittink, B. Knuiman, J. Derksen, V. Čapková, D. Twell… in Sexual Plant Reproduction (2000)

  6. No Access

    Chapter

    Acclimatization of Micropropagated Tobacco Plantlets

    Micropropagated plantlets are grown conventionally under conditions of low irradiance (30–70 μmol photons m-2 s-1), high relative air humidity, variable CO2 concentration (from 2% to compensation concentration) a...

    P. Kadleček, I. Tichá, V. Čapková, C. Schäfer in Photosynthesis: Mechanisms and Effects (1998)

  7. No Access

    Chapter

    Photosynthesis and Photoinhibition in Tobacco Plantlets Grown in vitro: An Ontogenetic Approach

    The specificity of plant in vitro growth is still not fully understood (1). For many reasons photoautotrophy has been favoured (2). But on the other hand the presence of sugars seemed to have a beneficial infl...

    I. Tichá, F. Čáp, D. Pacovská, D. Haisel… in Photosynthesis: Mechanisms and Effects (1998)

  8. No Access

    Article

    Protein synthesis in tobacco pollen tubes: preferential synthesis of cell-wall 69-kDa and 66-kDa glycoproteins

    One- and two-dimensional electrophoresis of Nicotiana tabacum pollen and pollen tube proteins confirmed that a new protein is preferentially synthesized during pollen germination and tube growth and becomes the m...

    V. Čapková, J. Tupý, J. Zbrožek in Sexual Plant Reproduction (1994)

  9. No Access

    Article

    Session 02 Hormone metabolism and action

    A. J. Boyd, S. M. Wahrlich, P. R. Poole, P. Brabcová, V. Čapková… in Biologia Plantarum (1994)

  10. No Access

    Article

    Protein synthesis in pollen tubes: preferential formation of new species independent of transcription

    Pollen tubes grown in vitro were pulselabelled for 5–30 min with [14C]amino acids, and the proteins analysed by SDS-PAGE and fluorography. In Nicotiana tabacum, the qualitative pattern of the synthesized proteins...

    V. Čapková, E. Hrabětová, J. Tupý in Sexual Plant Reproduction (1988)