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Article
Open AccessCardioprotective effects of early intervention with sacubitril/valsartan on pressure overloaded rat hearts
Left ventricular remodeling due to pressure overload is associated with poor prognosis. Sacubitril/valsartan is the first-in-class Angiotensin Receptor Neprilysin Inhibitor and has been demonstrated to have su...
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Article
Open AccessA predictive in vitro risk assessment platform for pro-arrhythmic toxicity using human 3D cardiac microtissues
Cardiotoxicity of pharmaceutical drugs, industrial chemicals, and environmental toxicants can be severe, even life threatening, which necessitates a thorough evaluation of the human response to chemical compou...
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Article
Narrow time window of metabolic changes associated with transition to overt heart failure in Tgaq*44 mice
The timing and consequences of alternations in substrate utilization in heart failure (HF) and their relationship with structural changes remain unclear. This study aimed to analyze metabolic changes associate...
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Article
An Improved Isolation Procedure for Adult Mouse Cardiomyocytes
Isolated adult mouse cardiomyocytes are an important tool in cardiovascular research, but are challenging to prepare. Because the energy supply determines cell function and viability, we compared total creatin...
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Article
NO and PGI2 in coronary endothelial dysfunction in transgenic mice with dilated cardiomyopathy
The aim of the present work was to analyze coronary endothelial function in the transgenic mouse model of dilated cardiomyopathy (Tgαq*44 mice).
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Article
Protein phosphorylation in isolated trabeculae from nonfailing and failing human hearts
Disturbances in the cAMP production during β-adrenergic stimulation and alterations of Ca 2+ transport controlling proteins and their regulation in the sarcoplasmic reticulum might be involved in the pathogenesis...
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Chapter
Protein phosphorylation in isolated trabeculae from nonfailing and failing human hearts
Disturbances in the cAMP production during β-adrenergic stimulation and alterations of Ca2+ transport controlling proteins and their regulation in the sarcoplasmic reticulum might be involved in the pathogenes...
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Article
Signal transduction pathway of the muscarinic receptors mediating gallbladder contraction
In gallbladder smooth muscle, carbachol interacts with M3 receptors to mediate contraction. To examine components of the intracellular second messenger system that is coupled to these receptors we have tested whe...
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Article
Isoprenaline-induced increase in the 40/41 kDa pertussis toxin substrates and functional consequences on contractile response in rat heart
Chronic β-adrenoceptor stimulation leads to desensitization of the myocardial adenylyl cyclase signalling pathway which includes β-adrenoceptor downregulation and upregulation of Gi-protein α-subunits. However, t...
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Article
Mechanism underlying the reduced positive inotropic effects of the phosphodiesterase III inhibitors pimobendan, adibendan and saterinone in failing as compared to nonfailing human cardiac muscle preparations
The present study was performed to compare the effects of the new positive inotropic phosphodiesterase III inhibitors pimobendan, adibendan, and saterinone on the isometric force of contraction in electrically...
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Article
Isoprenaline-induced increase in mRNA levels of inhibitory G-protein α-subunits in rat heart
Long-term β-adrenergic stimulation has been shown to desensitize the β-adrenoceptor/adenylyl cyclase signalling pathway at both the receptor and the G-protein level. To further elucidate the cellular mechanism...
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Article
Increased sensitivity to α-adrenoceptor stimulation but intact purinergic and muscarinergic effects in prehypertensive cardiac hypertrophy of spontaneously hypertensive rats
The effects of phenylephrine, isoprenaline and adenosine, (−)-N6-phenylisopropyladenosine (PIA) or carbachol alone and in the presence of isoprenaline on force of contraction were studied in isolated electrically...
