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Article
Feste Lipidnanopartikel zur Applikation unlöslicher Wirkstoffe
Solid lipid nanoparticles are promising carriers to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs). While the available amount of API, especially for newly identif...
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Article
Herstellung von Nanopartikeln zur Wirkstoffapplikation
A promising way to test poorly soluble active pharmaceutical ingredients (API) in cell culture or in animals is their application as nanoparticles. Dual centrifugation is a new technique to prepare those nanop...
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Article
Open AccessLipoprotein turnover and possible remnant accumulation in preeclampsia: insights from the Freiburg Preeclampsia H.E.L.P.-apheresis study
Preeclampsia is a life-threatening disease in pregnancy, and its complex pathomechanisms are poorly understood. In preeclampsia, lipid metabolism is substantially altered. In late onset preeclampsia, remnant r...
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Article
Open AccessDietary supplementation with n-3-fatty acids in patients with pancreatic cancer and cachexia: marine phospholipids versus fish oil - a randomized controlled double-blind trial
Like many other cancer patients, most pancreatic carcinoma patients suffer from severe weight loss. As shown in numerous studies with fish oil (FO) supplementation, a minimum daily intake of 1.5 g n-3-fatty ac...
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Article
Open AccessSaturated and mono-unsaturated lysophosphatidylcholine metabolism in tumour cells: a potential therapeutic target for preventing metastases
Metastasis is the leading cause of mortality in malignant diseases. Patients with metastasis often show reduced Lysophosphatidylcholine (LysoPC) plasma levels and treatment of metastatic tumour cells with satu...
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Article
Open AccessHealth effects of dietary phospholipids
Beneficial effects of dietary phospholipids (PLs) have been mentioned since the early 1900's in relation to different illnesses and symptoms, e.g. coronary heart disease, inflammation or cancer. This article g...
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Protocol
Tracing of siRNAs Inside Cells by FRET Imaging
The structural integrity of siRNA carrying fluorescent dyes attached to its different strands can be monitored through the efficiency of fluorescence resonance energy transfer between the dyes. In this chapte...
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Article
Marine phospholipids—a promising new dietary approach to tumor-associated weight loss
Advanced tumor disease very often evokes excessive loss of body weight. Among others, fish oil or marine fatty acid ethyl esters were investigated for treatment of cancer cachexia with controversial results. I...
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Protocol
FRET Imaging of Cells Transfected with siRNA/Liposome Complexes
By monitoring the efficiency of fluorescence resonance energy transfer of dyes attached to the different strands of siRNA, the structural integrity of the latter can be traced inside cells. Here, the experimen...
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Article
Anti-metastatic effects of liposomal gemcitabine in a human orthotopic LNCaP prostate cancer xenograft model
Fatal outcomes of prostate carcinoma (PCa) mostly result from metastatic spread rather than from primary tumor burden. Here, we monitored growth and metastatic spread of an orthotopic luciferase/GFP-expressing...
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Article
Open AccessPlasma lyso-phosphatidylcholine concentration is decreased in cancer patients with weight loss and activated inflammatory status
It has been observed that ras-transformed cell lines in culture have a higher phosphatidylcholine (PC) biosynthesis rate as well as higher PC-degradation rate (increased PC-turnover) than normal cells. In corr...
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Chapter
DNA-intercalators — the anthracyclines
The anthracyclines are derivatives of rhodomycin B, a red-pigmented polyketide antibiotic, isolated in the 1950s from Gram-positive Streptomyces present in an Indian soil sample. Many microorganisms produce and s...
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Article
Pharmacokinetics of liposomal doxorubicin (TLC-D99; Myocet) in patients with solid tumors: an open-label, single-dose study
Liposomal encapsulation of doxorubicin is designed to increase safety and tolerability by decreasing cardiac and gastrointestinal toxicity through decreased exposure of these tissues to doxorubicin, while effe...
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Article
Quantification of various phosphatidylcholines in liposomes by enzymatic assay
The purpose of this research was to adapt a colorimetric, phospholipase D-based serum-phospholipid assay for the quantification of phosphatidylcholine (PC) in liposomes using a microtitre plate reader. PC from...
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Article
Synthesis of Novel Thiol-Reactive Amphiphilic Lipids Based on Cholesterol for Protein-Liposome Coupling
The synthesis of a series of coupling lipids designed for covalently linking proteins to liposomes is described. The new compounds have in common a cholesterylsuccinyl unit as a lipid anchor and a thiol-react...
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Article
Synthesis of Isosteric Phosphono Analogs of Biologically Active Alkylphosphocholines
We describe a high yield reaction sequence for the conversion of long chain alcohols into 3-(trimethylammonio)propylphosphonates. The products are phospholipase D stable isosteric phosphono analogs of the res...
