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Article
Identification of a molecular signature predictive of sensitivity to differentiation induction in acute myeloid leukemia
Acute myeloid leukemia (AML) blasts are immature committed myeloid cells unable to spontaneously undergo terminal maturation, and characterized by heterogeneous sensitivity to natural differentiation inducers....
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Article
Gene expression profile of Vitamin D3 treated HL60 cells shows an incomplete molecular phenotypic conversion to monocytes
By high density oligonucleotide microarrays we have studied the expression profile of proliferating and VD treated HL60 cells and the molecular phenotype of VD monocytes and that of CD14+ peripheral monocytes ...
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Article
The effectiveness and tolerability of epoetin alfa in patients with multiple myeloma refractory to chemotherapy
Anemia is a frequent complication of multiple myeloma, becoming chronic in patients who are resistant to chemotherapy. This randomized, parallel, controlled multicenter study (71 patients receiving concomitant...
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Article
Sinus histiocytosis with massive lymphadenopathy: Immunological, cytogenetic and molecular studies
We describe a case of “sinus histiocytosis with massive lymphadenopathy” (SHML) studied by immunohistochemical, cytogenetic and molecular analysis. The immunophenoty** showed that the lymph node histiocytes ...
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Chapter
Heterogeneity of Human Normal and Leukemic Cells of Lymphoid and Myeloid Lineage in Respect to the Expression of c-myc and c-myb mRNA Transcripts
The clones pMC41.5 and pMC41.3, representing respectively the 5’ and the 3’ terminal of c-myc and the clone F8, representing the 3’ terminal of c-myb, were used to investigate the presence of myc and myb trans...
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Chapter
Transcripts of c-fes Oncogene are Present at Detectable Levels in Total Cellular RNA of Human Myeloid but not Lymphoid Normal and Neoplastic Cells
Total cellular RNA from a variety of normal and leukemic myeloid and lymphoid cell populations was analysed for the expression of a human cellular oncogene, c-fes, by Northern blot hybridization assays. The pr...
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Chapter
Detection of c-myc, c-myb and c-fes Oncogene Transcripts in Human Myeloid and Lymphoid Normal and Leukemic Cells by in Situ Hybridization
The in situ hybridization technique was used to investigate the expression of the human cellular oncogenes, c-myc, c-myb and c-fes, simultaneously on different cell populations: normal resting and PHA stimulat...
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Chapter and Conference Paper
Different Frequency Classes of Sequences in Heterogeneous Nuclear RNA of Normal Promyelocytes and Lymphoblasts and of Leukemic Blast Cells of Circulating Blood and of the HL60 Line
A number of studies have led to the conclusion that each differentiated cell nucleus includes not only all of the genes ever utilized in the organism but also transcripts of most of these genes. A direct impli...
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Article
Kinetics of hybridization to human DNA of heterogeneous nuclear RNA isolated from normal human lymphoblasts and acute leukemia blast cells
Heterogeneous nuclear RNA was extracted from normal PHA-stimulated human lymphocytes and acute myeloid leukemia blast cells. Experiments were performed to determine the hybridization kinetics of these RNAs to ...
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Article
Double stranded ribonuclease activity in human lymphocyte nuclei
Ribonuclease activity directed against the synthetic duplex polyrC: polyrI was detected in nuclear extracts from both unstimulated and PHA-stimulated human lymphocytes. In the latter cells, the activity was ab...
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Article
An Unstable Ribonucleic Acid in Normal Human Erythroblasts
IT is now well established that the primary information required for protein synthesis is sited on DNA. The mechanism whereby, in animal cells, this information is conveyed and elaborated, so that protein synt...
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Article
Differences in the dexoxyribonucleoprotein complex of normal and leukemic human lymphocytes
Gli autori hanno determinato con metodo citofotometrico nel visibile il contenuto di acido desossiribonucleico (DNA) e di proteina istonica nei linfociti di 4 soggetti normali e di 4 pazienti affetti da leucos...
