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Article
Comparative studies on the primary structure of acetylcholinesterases from bovine caudate nucleus and bovine erythrocytes
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Comparison of partial amino acid sequences of G2-acetylcholinesterase (AChE) from bovine erythrocytes and G4-...
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Article
Reduced myelinogenesis and recovery in hyperphenylalaninemic rats
In a previous paper (Burri et al., 1990), we have shown that experimental hyperphenylalaninemia (hyper-Phe) in 3–17 d-old rats leads to reduced myelinogenesis. Such treated rats recover during a 6 w low phenyl...
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Article
A sensitive protein assay method using micro-titer plates
A commercially available protein-assay, based on the reaction between bicinchoninic acid (4,4′-dicarboxy-2,2′-biquinoline) and copper was adapted for use in microtiter plates. The assay is sensitive between 10...
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Chapter and Conference Paper
Multiple Molecular Forms of Acetylcholinesterase and Their Possible Role in the Degradation of Neurohormones
The role of acetylcholinesterase (AChE; EC 3.1.1.7) is well established. It inactivates by hydrolysis acetylcholine, the transmitter substance operating in cholinergic neurotransmission. Besides its activity a...
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Chapter
Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmorata by Charge Shift Crossed Immunoelectrophoresis
The electric organ of Torpedo marmorata contains two different classes of acetylcholinesterase (AChE) (for review see Massoulie and Bon 1982) which can be distinguished by their solubilization and sedimentation b...
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Chapter
Reconstitution of Pure Acetylcholine Receptor
The procedure for the isolation and the purification of the acetylcholine receptor from the electric organ of Torpedo marmorata has been described in Chapter 2.3. Reconstitution of the acetylcholine receptor in a...
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Chapter
Purification by Affinity Chromatography of Red Cell Membrane Acetylcholinesterase
Over the past the methods for purifying integral membrane proteins have advanced considerably. In particular the onset of affinity chromatography (Jakoby and Wilchek 1974) has brought new incentives to this pa...
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Chapter
Crosslinking of an Amphiphilic Protein: Human Erythrocyte Membrane Acetylcholinesterase
Crosslinking has become a method widely used to study the composition and symmetry of oligomeric proteins (Wold 1972; Peters and Richards 1977; Ji 1979; Das and Fox 1979). In this experiment an imidate and glu...
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Chapter
Use of a Potentiometric Cyanine Dye in the Study of Reconstituted Membrane Proteins
The reconstitution procedure described on p. 103 of this book yields phospholipid vesicles with an outside diameter of 25–30 nm. The membrane protein of interest is contained in a single bilayer structure, whi...
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Chapter
Preparation of Spectrin-Free Vesicles from Human Red Blood Cells
An important prerequisite in the study of erythrocyte membrane structure and function is the availability of suitable membrane preparations. Several methods by which such “ghosts” can be prepared have been pub...