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  1. No Access

    Protocol

    Protein Purification and Digestion Methods for Bacterial Proteomic Analyses

    Reproducible protein extraction is critical for the quantitative analysis of bacterial proteomes. While a wide range of techniques exist, there is no one-size-fits-all solution that will be suitable for all ap...

    Nicole Hansmeier, Samrachana Sharma, Tzu-Chiao Chao in Proteomics in Systems Biology (2022)

  2. No Access

    Protocol

    Affinity Enrichment of Salmonella-Modified Membranes from Murine Macrophages for Proteomic Analyses

    Dissecting host–pathogen interaction requires the ability to specifically enrich distinct proteins along with their co-assembled constituents or complexes. Affinity technologies leverage specificity of reagent...

    Tzu-Chiao Chao, Samina Thapa, Nicole Hansmeier in Proteomics in Systems Biology (2022)

  3. Article

    Open Access

    Re-engineering cellular physiology by rewiring high-level global regulatory genes

    Knowledge of global regulatory networks has been exploited to rewire the gene control programmes of the model bacterium Salmonella enterica serovar Typhimurium. The product is an organism with competitive fitness...

    Stephen Fitzgerald, Shane C. Dillon, Tzu-Chiao Chao… in Scientific Reports (2015)

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  4. No Access

    Article

    Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

    Femtosecond X-ray pulses were used to obtain diffraction data on photosystem II, revealing conformational changes as the complex transitions from the dark S1 state to the double-pumped S3 state; the time-resolved...

    Christopher Kupitz, Shibom Basu, Ingo Grotjohann, Raimund Fromme… in Nature (2014)

  5. No Access

    Article

    Selective trap** of single mammalian breast cancer cells by insulator-based dielectrophoresis

    The trap** or immobilization of individual cells at specific locations in microfluidic platforms is essential for single cell studies, especially those requiring cell stimulation and downstream analysis of c...

    Sanchari Bhattacharya, Tzu-Chiao Chao… in Analytical and Bioanalytical Chemistry (2014)

  6. No Access

    Article

    Beyond nC60: strategies for identification of transformation products of fullerene oxidation in aquatic and biological samples

    Owing to their exceptional properties and versatility, fullerenes are in widespread use for numerous applications. Increased production and use of fullerenes will inevitably result in accelerated environmental...

    Benny F. G. Pycke, Tzu-Chiao Chao, Pierre Herckes… in Analytical and Bioanalytical Chemistry (2012)

  7. No Access

    Article

    Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry

    The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell p...

    Mian Yang, Tzu-Chiao Chao, Randall Nelson… in Analytical and Bioanalytical Chemistry (2012)