21 Result(s)
within
Tomasz J. Nowakowski
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Large-scale neurophysiology and single-cell profiling in human neuroscience
Advances in large-scale single-unit human neurophysiology, single-cell RNA sequencing, spatial transcriptomics and long-term ex vivo tissue culture of surgically resected human brain tissue have provided an un...
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Open AccessThe development and evolution of inhibitory neurons in primate cerebrum
Neuroanatomists have long speculated that expanded primate brains contain an increased morphological diversity of inhibitory neurons (INs)1, and recent studies have identified primate-specific neuronal population...
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Open AccessSingle-cell delineation of lineage and genetic identity in the mouse brain
During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The...
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Individual human cortical progenitors can produce excitatory and inhibitory neurons
The cerebral cortex is a cellularly complex structure comprising a rich diversity of neuronal and glial cell types. Cortical neurons can be broadly categorized into two classes—excitatory neurons that use the ...
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Open AccessPicroscope: low-cost system for simultaneous longitudinal biological imaging
Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomp...
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Open AccessAn atlas of cortical arealization identifies dynamic molecular signatures
The human brain is subdivided into distinct anatomical structures, including the neocortex, which in turn encompasses dozens of distinct specialized cortical areas. Early morphogenetic gradients are known to e...
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Open AccessSingle-cell epigenomics reveals mechanisms of human cortical development
During mammalian development, differences in chromatin state coincide with cellular differentiation and reflect changes in the gene regulatory landscape1. In the develo** brain, cell fate specification and topo...
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Distinct nuclear compartment-associated genome architecture in the develo** mammalian brain
Nuclear compartments are thought to play a role in three-dimensional genome organization and gene expression. In mammalian brain, the architecture and dynamics of nuclear compartment-associated genome organiza...
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Open AccessSingle-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia
The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of h...
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Cell stress in cortical organoids impairs molecular subtype specification
Cortical organoids are self-organizing three-dimensional cultures that model features of the develo** human cerebral cortex1,2. However, the fidelity of organoid models remains unclear3–5. Here we analyse the t...
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Open AccessDisruptive mutations in TANC2 define a neurodevelopmental syndrome associated with psychiatric disorders
Postsynaptic density (PSD) proteins have been implicated in the pathophysiology of neurodevelopmental and psychiatric disorders. Here, we present detailed clinical and genetic data for 20 patients with likely ...
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Open AccessAutomated four-dimensional long term imaging enables single cell tracking within organotypic brain slices to study neurodevelopment and degeneration
Current approaches for dynamic profiling of single cells rely on dissociated cultures, which lack important biological features existing in tissues. Organotypic slice cultures preserve aspects of structural an...
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Regulation of cell-type-specific transcriptomes by microRNA networks during human brain development
MicroRNAs (miRNAs) regulate many cellular events during brain development by interacting with hundreds of mRNA transcripts. However, miRNAs operate nonuniformly upon the transcriptional profile with an as yet ...
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Open AccessIdentification of cell types in a mouse brain single-cell atlas using low sampling coverage
High throughput methods for profiling the transcriptomes of single cells have recently emerged as transformative approaches for large-scale population surveys of cellular diversity in heterogeneous primary tis...
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An analytical framework for whole-genome sequence association studies and its implications for autism spectrum disorder
Genomic association studies of common or rare protein-coding variation have established robust statistical approaches to account for multiple testing. Here we present a comparable framework to evaluate rare an...
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Open AccessThe impact of microRNAs on transcriptional heterogeneity and gene co-expression across single embryonic stem cells
MicroRNAs act posttranscriptionally to suppress multiple target genes within a cell population. To what extent this multi-target suppression occurs in individual cells and how it impacts transcriptional hetero...
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Open AccessSingle-cell analysis of long non-coding RNAs in the develo** human neocortex
Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- and tissue-specific expres...
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Radial glia require PDGFD–PDGFRβ signalling in human but not mouse neocortex
The transcriptional profiles of radial glia are compared between humans and mice during neurogenesis, implicating the growth factor PDGFD and its receptor, PDGFRβ, in human but not mouse neocortical development.
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Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in develo** cerebral cortex
Pollen et al. show that low-coverage RNA sequencing of single cells is a powerful approach for characterizing heterogeneous cell populations.
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Open AccessThe expression and activity of β-catenin in the thalamus and its projections to the cerebral cortex in the mouse embryo
The mammalian thalamus relays sensory information from the periphery to the cerebral cortex for cognitive processing via the thalamocortical tract. The thalamocortical tract forms during embryonic development ...
