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Article
Open AccessUnravelling the Role of O-glycans in Influenza A Virus Infection
The initial stage of host cell infection by influenza A viruses (IAV) is mediated through interaction of the viral haemagglutinin (HA) with cell surface glycans. The binding requirement of IAVs for Galβ(1,4)Gl...
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Article
Open AccessStructural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt’s lymphoma (BL) Daudi cells by NMR spectroscopy
Siglec-2 undergoes constitutive endocytosis and is a drug target for autoimmune diseases and B cell-derived malignancies, including hairy cell leukaemia, marginal zone lymphoma, chronic lymphocytic leukaemia a...
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Article
Functional and structural characterization of a heparanase
Structure and functional characterization of BpHep, a heparanase from the invasive pathogenic bacterium Burkholderia pseudomallei, defines its glycosaminoglycan recognition mechanism and its catalytic mechanism a...
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Article
Revisiting the role of histo-blood group antigens in rotavirus host-cell invasion
Histo-blood group antigens (HBGAs) have been proposed as rotavirus receptors. H type-1 and Lewisb antigens have been reported to bind VP8* from major human rotavirus genotypes P[4], P[6] and P[8], while VP8* from...
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Reference Work Entry In depth
Influenza Virus, Overview: Structures, Infection Mechanisms and Antivirals
Hemagglutinin (HA) and neuraminidase (NA) are the two major surface proteins of influenza A virus (IAV). Initial attachment of the virus to the host cell is mediated by the binding of terminal sialic acids (Si...
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Article
Open AccessFerrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors
Mammals express the sialic acids N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) on cell surfaces, where they act as receptors for pathogens, including influenza A virus (IAV). Neu5Gc is s...
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Article
Structure-guided discovery of potent and dual-acting human parainfluenza virus haemagglutinin–neuraminidase inhibitors
Human parainfluenza viruses (hPIVs) cause upper and lower respiratory tract disease in children that results in a significant number of hospitalizations and impacts health systems worldwide. To date, neither a...
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Living Reference Work Entry In depth
Influenza Virus, Overview: Structures, Infection Mechanisms and Antivirals
Hemagglutinin (HA) and neuraminidase (NA) are the two major surface proteins of influenza A virus (IAV). Initial attachment of the virus to the host cell is mediated by the binding of terminal sialic acids (Si...
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Article
Production of α-L-iduronidase in maize for the potential treatment of a human lysosomal storage disease
Lysosomal storage diseases are a class of over 70 rare genetic diseases that are amenable to enzyme replacement therapy. Towards develo** a plant-based enzyme replacement therapeutic for the lysosomal storag...
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Article
Influence of an ER-retention signal on the N-glycosylation of recombinant human α-l-iduronidase generated in seeds of Arabidopsis
Processes associated with late events of N-glycosylation within the plant Golgi complex are a major limitation to the use of plant-based systems to produce recombinant pharmaceutical proteins for parenteral ad...
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Article
Sialic acid dependence in rotavirus host cell invasion
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Protocol
Saturation Transfer Difference NMR Spectroscopy as a Technique to Investigate Protein-Carbohydrate Interactions in Solution
Saturation transfer difference (STD) Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful method for studying protein–ligand interactions in solution. The STD NMR method is capable of identifying the bi...
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Article
Influenza C virus and bovine coronavirus esterase reveal a similar catalytic mechanism: new insights for drug discovery
Both, the influenza C (INF-C) virus haemagglutinin esterase fusion and bovine coronavirus (BCoV) haemagglutinin esterase surface glycoproteins exhibit a lectin binding capability and a receptor-destroying 9-O-ace...
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Article
A 1H STD NMR spectroscopic investigation of sialylnucleoside mimetics as probes of CMP-Kdn synthetase
CMP-Kdn synthetase catalyses the reaction of sialic acids (Sia) and CTP to the corresponding activated sugar nucleotide CMP-Sia and pyrophosphate PP i . Saturation Transfer Diffe...
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Article
Conformational analysis of a Chlamydia-specific disaccharide α-Kdo-(2→8)-α-Kdo-(2→O)-allyl in aqueous solution and bound to a monoclonal antibody: Observation of intermolecular transfer NOEs
The disaccharide α-Kdo-(2→8)-α-Kdo (Kdo: 3-deoxy-d-manno-oct-2-ulosonic acid) represents a genus-specific epitope of the lipopolysaccharide of the obligate intracellular human pathogen Chlamydia. The conformat...
