-
Chapter and Conference Paper
TAC–TICS: TRANSPOSON-BASED BIOLOGICAL PEST MANAGEMENT SYSTEMS
A system in which a specific pest insect population is targeted for management by making that population susceptible to control is described. The four key components of this genetic control system are: (a) tra...
-
Chapter and Conference Paper
FUNCTIONAL GENOMICS: FUNCTIONAL RECONSTITUTION OF PORTIONS OF THE PROTEOME IN INSECT CELL-LINES
This chapter describes the assembly and use of a gene expression system that allows a wide variety of proteins to be cloned and expressed in insect cell-lines grown in culture. The system faithfully produces s...
-
Article
Identification of three histone methyltransferases in Drosophila: dG9a is a suppressor of PEV and is required for gene silencing
Organization of chromatin structure and regulation of gene transcription are contingent on histone tail modifications. Regions of the genome packaged with nucleosomes that contain methyl histone H3 at lysine 9...
-
Article
Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture
Factor Xa is a serine protease, whose high selectivity can be used to cleave protein tags from recombinant proteins. A fusion protein comprised of a self-activating form of factor X linked to a cellulose-bindi...
-
Article
Mutations in the nomad retroelement are modifiers of position-effect variegation in Drosophila melanogaster
The E(var)63A P mutation of Drosophila melanogaster was isolated in a genetic screen for P-element induced enhancers of w m4 variegation. Remobilization of the P-el...
-
Article
Characterization of mutations that enhance position-effect variegation in Drosophila melanogaster
Several mutants that enhance the gene inactivation associated with position-effect variegation [E(var) mutants] have been characterized. These include three ethyl methanesulfonate (EMS)-induced lesions and a seco...
-
Chapter
Programmed Cell Death and Aging in Drosophila Melanogaster
While a variety of theories regarding the etiology of aging have been proposed, they can be classified under two extreme models. One general model asserts that senescence is the result of attrition caused by n...
-
Article
Sequences homologous to the P mobile element of Drosophila melanogaster are widely distributed in the subgenus Sophophora
The biological properties of the P element of Drosophila melanogaster differ from those of other mobile elements in this genus in that its mobility is associated with dysgenic traits that might be expected to lim...
-
Article
Regulation of dosage compensation in X-chromosomal trisomies of Drosophila melanogaster
In this study, we provide evidence which supports the possibility that dosage compensation in X-chromosomal trisomies (metafemales) can occur by a mechanism distinct from the regulatory system that operates to...
-
Article
Dosage compensation is transcriptionally regulated in autosomal trisomies of Drosophila
Drosophila melanogaster that are trisomic for an entire chromosome arm can survive to late stages of development. Some 2L-linked enzyme loci display diploid levels of gene product in trisomic-2L larvae. Measurem...
-
Article
Genes which suppress position-effect variegation in Drosophila melanogaster are clustered
We have isolated more than 50 dominant suppressors and 3 dominant enhancers of position effect variegation in Drosophila melanogaster. To our surprise, genetic map** studies revealed that the Su(var) mutations ...
-
Article
RNA-DNA hybridization analyses of tRNA 3b Val in Drosophila melanogaster
Transfer RNA was extracted from 50–300 mg of adult flies and specifically labeled in vitro. The level of individual isoacceptors was quantitated by efficient annealing to Drosphila tRNA genes carried on recombina...
-
Article
Histone gene deficiencies and position–effect variegation in Drosophila
Position–effect variegation, originally observed in insects, has since been demonstrated in plants and mammals. When cells bear a chromosomal rearrangement which juxtaposes a euchromatic gene near to heterochr...
-
Article
Temperature-sensitive mutations in Drosophila melanogaster
Improved methods for rearing and screening large numbers of flies permitted the recovery of 10 mutations exhibiting a reversible temperature-dependent adult paralysis among 1.1×106 flies tested. Of the 10 mutatio...