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Article
Open AccessFibrinolysis-resistant carbonylated fibrin detected in thrombi attached to the vascular wall of abdominal aortic aneurysms
In this study, we investigated how carbonylation of fibrinogen by acrolein modified its indispensable function to enhance fibrinolysis after being converted to fibrin and contributed to generating a fibrinolys...
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Article
Open AccessAdipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm
Abdominal aortic aneurysm (AAA) is a vascular disease involving the gradual dilation of the abdominal aorta. It has been reported that development of AAA is associated with inflammation of the vascular wall; h...
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Article
In vivo imaging analysis of the interaction between unusually large von Willebrand factor multimers and platelets on the surface of vascular wall
To elucidate how unusually large von Willebrand factor (UL-VWF) multimers facilitate thrombus formation, their behavior was analyzed together with that of platelets in living mice deficient in the gene encodin...
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Article
A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy
Physiological phenomena are regulated by multiple signal pathways upon receptor stimulation. Here, we have introduced a new technique with a combination of prism-based total internal reflection fluorescence mi...
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Article
Enhancement of fibrinolysis by gel-filtered platelets and its quenching by cytochalasin B and GPIIb/IIIa antagonists
The effects of gel-filtered platelets on euglobulin clot lysis time (ECLT) were analyzed to elucidate the possible role of platelets in thrombolysis. Gel-filtered platelet-supplemented ECLT (plt-ECLT) was sign...
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Article
Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo
The exact mechanism of blood vessel thrombus formation remains to be defined. Here, we introduce a new approach to probe thrombus formation in blood vessels of living animals using intravital microscopy in gre...
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Chapter
Role of tPA in the Neural System
In addition to triggering fibrinolysis cascades via plasminogen activation, recent studies have revealed new functions for the tissue plasminogen activator (tPA) in the neural system. In the central nervous sy...
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Article
Postoperative changes in plasma tissue-type plasminogen activator and type I plasminogen activator inhibitor
To clarify the changes which occur postoperatively in intravascular fibrinolysis, plasma levels of tissue-type plasminogen activator (t-PA) antigen, the total plasminogen activator inhibitor type-1 (PAI-1) ant...
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Chapter and Conference Paper
Conformational Change of Glu-Plasminogen: Effects of N-Terminal Peptides of Glu-Plasminogen
Native plasminogen with glutamic acid at the N-terminus (Glu-plg) has a tight conformation possibly due to the binding of the N-terminal part to the lysine binding sites (LBS) on kringle 1 (1, 2) or kringle 4 ...
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Chapter and Conference Paper
The Cleavage of a Serine Protease Inhibitor at the Reactive Center by Its Target Protease: Analysis of the Substrate-Like Form of a Serpin
Many enzymes involved in coagulation and fibrinolysis have a serine residue at their active site and they are therefore defined as serine proteases. In plasma the activity of such proteases are controlled by s...
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Chapter and Conference Paper
Effect of Calcium Ion on the Euglobulin Clot Lysis Time: Significant shortening by physiological concentration of calcium ion
Euglobulin clot lysis time (ECLT) assay has been considered to be useful to assess the systemic fibrinolytic activity [1] [2]. ECLT is respected to show mainly the activity of tissue plasminogen activator (tPA...
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Chapter and Conference Paper
The Dissociation of α2-Plasmin-Inhibitor-Plasmin Complex to Active Plasmin by SDS Treatment
α2-PI has been demonstrated to be the physiologically most important inhibitor to plas. sin in plasma [1–3], it is a single-chain glycoprotein with molecular weight of about 68,000 [2,4] and inhibits proteolyt...
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Chapter and Conference Paper
Effects of Various N-Terminal Peptides of Glu-Plasminogen on Kee** Its Tight Conformation of Glu-Plasminogen
Glu-plasminogen (Glu-plg) is a single chain plasma glycoprotein, which is the zymogen form of a serine protease, plasmin. The conversion from the precursor to the enzyme occurs as a result of cleavage of the Arg5...
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Article
Influence of various structural domains of fibrinogen and fibrin on the potentiation of plasminogen activation by recombinant tissue plasminogen activator
Fibrinogen, fibrin, and related fragments have varying stimulatory effects on the initial rate of the activation of human plasminogen ([Glu1]Pg) by recombinant tissue plasminogen activator (rt-PA). A detailed ana...
