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Article
Open AccessMultiple circulating forms of neprilysin detected with novel epitope-directed monoclonal antibodies
Neprilysin (NEP) is an emerging biomarker for various diseases including heart failure (HF). However, major inter-assay inconsistency in the reported concentrations of circulating NEP and uncertainty with resp...
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Article
Open AccessProteomic analysis of ceftazidime and meropenem-exposed Pseudomonas aeruginosa ATCC 9027
Pseudomonas aeruginosa is well known for its intrinsic ability to resist a wide range of antibiotics, thus complicates treatment. Thus, understanding the response of the pathogen to antibiotics is important for d...
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Open AccessAuthor Correction: Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
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Article
Open AccessEpitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
High quality, well-validated antibodies are needed to mitigate irreproducibility and clarify conflicting data in science. We describe an epitope-directed monoclonal antibody (mAb) production method that addres...
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Article
Open AccessPyrazinamide triggers degradation of its target aspartate decarboxylase
Pyrazinamide is a sterilizing first-line tuberculosis drug. Genetic, metabolomic and biophysical analyses previously demonstrated that pyrazinoic acid, the bioactive form of the prodrug pyrazinamide (PZA), int...
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Article
In-depth proteomic profiling of the Singapore grouper iridovirus virion
Singapore grouper iridovirus (SGIV) is a lethal grouper virus containing 162 predicted ORFs. Previous proteomic studies led to identification of 73 SGIV structural proteins. Here, SDS-assisted tube-gel digesti...
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Article
Open AccessYeast aconitase mitochondrial import is modulated by interactions of its C and N terminal domains and Ssa1/2 (Hsp70)
Molecules of single proteins, echoforms, can be distributed between two (or more) subcellular locations, a phenomenon which we refer to as dual targeting or dual localization. The yeast aconitase gene ACO1 (778 a...
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Article
Open AccessCorrection: Corrigendum: Stk38 Modulates Rbm24 Protein Stability to Regulate Sarcomere Assembly in Cardiomyocytes
Scientific Reports 7: Article number: 44870; published online: 21 March 2017; updated: 30 June 2017. The original version of this Article contained errors. The original version contained errors in the spelling...
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Open AccessStk38 Modulates Rbm24 Protein Stability to Regulate Sarcomere Assembly in Cardiomyocytes
RNA-binding protein Rbm24 is a key regulator of heart development and required for sarcomere assembly and heart contractility. Yet, its underlying mechanism remains unclear. Here, we link serine/threonine kina...
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Article
Open AccessComprehensive and quantitative proteomic analyses of zebrafish plasma reveals conserved protein profiles between genders and between zebrafish and human
Omic approaches have been increasingly used in the zebrafish model for holistic understanding of molecular events and mechanisms of tissue functions. However, plasma is rarely used for omic profiling because o...
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Article
Open AccessIn situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line
To date, the exact targets and mechanism of action of curcumin, a natural product with anti-inflammatory and anti-cancer properties, remain elusive. Here we synthesized a cell permeable curcumin probe (Cur-P) wit...
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Article
Open AccessHaem-activated promiscuous targeting of artemisinin in Plasmodium falciparum
The mechanism of action of artemisinin and its derivatives, the most potent of the anti-malarial drugs, is not completely understood. Here we present an unbiased chemical proteomics analysis to directly explor...
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Article
Open AccessMap** sites of aspirin-induced acetylations in live cells by quantitative acid-cleavable activity-based protein profiling (QA-ABPP)
Target-identification and understanding of mechanism-of-action (MOA) are challenging for development of small-molecule probes and their application in biology and drug discovery. For example, although aspirin ...
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iTRAQ™ Labeling Coupled with LC-MALDI Mass Spectrometry for Monitoring Temporal Response of Colorectal Cancer Cells to Butyrate Treatment
Mass spectrometry (MS)-based quantitative proteomics plays important roles in drug discovery. In this chapter, we describe a stable isotope labeling technique which employs 4-plex iTRAQ™ isobaric reagents coupled...
