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Article
Detection of four isomers of the human cytomegalovirus genome using nanopore long-read sequencing
Human cytomegalovirus has a linear DNA genome with a total length of approximately 235 kb. This large genome is divided into two domains, "Long" and "Short". There are four isomers of the cytomegalovirus genom...
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Article
Open AccessIdentification and clinical significance of somatic oncogenic mutations in epithelial ovarian cancer
Epithelial ovarian cancer (EOC) is a heterogeneous disease with diverse clinicopathological features and behaviors, and its heterogeneity may be concerned with the accumulation of multiple somatic oncogenic mu...
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Article
Open AccessClinical implication of oncogenic somatic mutations in early-stage cervical cancer with radical hysterectomy
It is well known that tumour initiation and progression are primarily an accumulation of genetic mutations. The mutation status of a tumour may predict prognosis and enable better selection of targeted therapi...
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Article
Tumor mutation burden and immunological, genomic, and clinicopathological factors as biomarkers for checkpoint inhibitor treatment of patients with non-small-cell lung cancer
Cancer treatment using immune checkpoint inhibitors is widely used, although biomarkers predictive of response are not well established. However, both the expressions of programmed cell death ligand 1 (PD-L1) ...
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Article
Human protein factory for converting the transcriptome into an in vitro–expressed proteome
A collection of 33,275 human Gateway entry clones and complementary in vitro protein expression methodologies are described that allow proteome-scale production of human proteins. This 'human protein factory' was...
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Article
Complete sequencing and characterization of 21,243 full-length human cDNAs
As a base for human transcriptome and functional genomics, we created the “full-length long Japan” (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and fou...
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Article
Salusins: newly identified bioactive peptides with hemodynamic and mitogenic activities
The discovery of endogenous bioactive peptides has typically required a lengthy identification process. Computer-assisted analysis of cDNA and genomic DNA sequence information can markedly shorten the process....
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Article
Use of sample sequencing to identify candidate genes in the cystinosis critical region of chromosome 17p13 • 618
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Article
Construction of a 7-Aminocephalosporanic Acid (7ACA) Biosynthetic Operon and Direct Production of 7ACA in Acremonium Chrysogenum
We have used cDNA encoding D-amino acid oxidase, and genomic DNA encoding cephalosporin acylase from Fusarium solani and Pseudomonas diminuta, respectively, to construct a novel hybrid 7-aminocephalosporanic acid...
