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Article
Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum
Chlorosomes are large and efficient light-harvesting organelles in green photosynthetic bacteria, and they characteristically contain large numbers of bacteriochlorophyll c, d, or e molecules. Self-aggregated bac...
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Article
Isolation and characterization of a new bacteriochlorophyll-c bearing a neopentyl substituent at the 8-position from the bciD-deletion mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum
We recently constructed the mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum lacking BciD which was responsible for formation of a formyl group at the 7-position in bacteriochlorophyll(BC...
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Article
Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum
The mutant lacking enzymes BciA and BchU, that catalyzed reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobacu...
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Article
A variety of glycolipids in green photosynthetic bacteria
The compositions of glycolipids in the following seven strains of green photosynthetic bacteria were investigated at the molecular level using LC–MS coupled with an evaporative light scattering detector: Chlorobi...
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Article
The pigment stoichiometry in a chlorophyll a/c type photosynthetic antenna
The trimeric fucoxanthin–chlorophyll a/c protein (FCP) was purified from a Japanese brown alga, Cladosiphon okamuranus TOKIDA. Its pigment stoichiometry was determined to be chlorophyll (Chl) a:Chl c ...
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Article
In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing organisms
Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of th...
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Article
Synthesis of 18O-labeled photosynthetically active chlorophylls at the 3- or 7-carbonyl group with high regioselectivity
The 3- and 7-formyl groups of chlorophyll-d (Chl-d) and bacteriochlorophyll-e (BChl-e), respectively, were regioselectively labeled with an isotopically stable oxygen-18 (18O) atom to give 31-18O-labeled Chl-d an...
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Article
Composition and localization of bacteriochlorophyll a intermediates in the purple photosynthetic bacterium Rhodopseudomonas sp. Rits
Rhodopseudomonas sp. Rits is a recently isolated new species of photosynthetic bacteria and found to accumulate a significantly high amount of bacteriochlorophyll (BChl) a intermediates possessing non-, di- and t...
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Article
Central-Cis isomers of lutein found in the major light-harvesting complex of Photosystem II (LHC IIb) of higher plants
Lutein (β,∈-carotene-3,3′-diol) is the major carotenoid of the light-harvesting systems of higher plants. Lutein was isolated at 4°C and in complete darkness from the bulk light-harvesting complex of Photosyst...
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Chapter
Stacking of Macrocycles in Bacteriochlorophyll c Aggregates and in Chlorosomes
Green photosynthetic bacteria have an antenna complex called ‘chlorosomes’ that contain bacteriochlorophyll c (BChl c) as the major component, and the self-assembly of the BChl c molecules is supposed to play ...
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Article
Continuous antibiotic production by an immobilized cyanobacterium in a seaweed-type bioreactor
The filamentous cyanobacterium,Scytonema sp. TISTR 8208, which produces a cyclic peptide antibiotic, was cultivated for 20 d in a seaweed-type bioreactor containing anchored polyurethan foam strips. Cells immobil...
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Article
Antibiotic production by the immobilized cyanobacterium,Scytonema sp. TISTR 8208, in a seaweed-type photobioreactor
A photobioreactor was constructed using either anchored polyurethane foam strips (1 × 1 × 40 cm, PU-strips) fixed on a stainless-steel ring to prevent flotation, or free-floating polyurethane foam blocks (1 × ...