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Article
NAC-1, a potential stem cell pluripotency factor, contributes to paclitaxel resistance in ovarian cancer through inactivating Gadd45 pathway
Nucleus accumbens-1 (Nac1 or NAC-1) belongs to the BTB/POZ (Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad complex) transcription factor family and is a novel protein that potentially participates in se...
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Article
Determination of Volatile Carbonyl Compounds in Cigarette Smoke by LC-DAD
An effective and rapid method, use of a 2,4-dinitrophenylhydrazine (DNPH)-treated Cambridge filter and high-performance liquid chromatography (HPLC) with diode-array detection (DAD), has been used for determin...
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Article
Recombinant DNA vaccines protect against tumors that are resistant to recombinant vaccinia vaccines containing the same gene
Antigen-specific cancer immunotherapy involves the delivery of tumor-associated antigen to the host for the generation of tumor-specific immune responses and antitumor effects. We hypothesized that different d...
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Article
Intramuscular administration of E7-transfected dendritic cells generates the most potent E7-specific anti-tumor immunity
Dendritic cells (DCs) are highly efficient antigen-presenting cells capable of priming both cytotoxic and helper T cells in vivo. Recent studies have demonstrated the potential use of DCs that are modified to ...
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Article
Blue light but not red light induces a calcium transient in the moss Physcomitrella patens (Hedw.) B., S. & G.
In caulonemal filaments of the moss, Physcomitrella patens, which had been incubated in darkness, 3 s irradiation with blue light (λmax 450 nm) at fluence rates of 100 μmol m−2 s−1 and above caused a transient␣i...
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Chapter
Down Regulation of Nodule Sucrose Synthase by Mutation and Antisense
Legume nodules are entirely dependent on photosynthetic sucrose to fuel N2 fixation. Nodule cells have two enzymes capable of metabolizing sucrose, alkaline invertase (AI) and sucrose synthase (SS). The fact that...
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Article
Allozyme variation in populations, full-sib families and selfed lines in Betula pendula Roth
Changes in genetic variability in populations (stand origins), full-sib (FS) families and three generations of selfed lines of Betula pendula were observed based on 15 allozyme loci. Growth vigour, measured as st...
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Article
The moss,Physcomitrella patens, transformed with apoaequorin cDNA responds to cold shock, mechanical perturbation and pH with transient increases in cytoplasmic calcium
The gene for apoaequorin has been used previously to indicate cytosolic calcium changes in higher plants. Here we report the transformation of the mossPhyscomitrella patens with the cDNA for apoaequorin. Stable t...
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Article
Growth performance of hybrid families by crossing selfed lines of Betula pendula Roth
Hybrid breeding is an effective approach in many agricultural crops. In allogamous tree species severe inbreeding depression and long reproductive cycles generally prohibit its use. However, three generations ...
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Chapter
Gif Chemistry
The selective functionalization of saturated hydrocarbons is an important and challenging academic and industrial objective. Our own contributions to this area are based on Gif-type systems. Recent studies hav...
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Article
An analysis of seed development inPisum sativum L.
Indirect immunofluorescence has been used to examine the temporal and spatial distribution of cells containing the storage protein vicilin, in immature pea embryos grown in vivo and in culture. The occurrence ...
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Article
An analysis of seed development inPisum sativum L.
Pea embryos have been treated with xenobiotic substances to manipulate specific cellular events. Taxol was able to induce condensation of chromatin in endoreduplicated nuclei, resulting in cells with either no...
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Chapter and Conference Paper
Developmental Studies of Physcomitrella patens Using Auxin and Cytokinin Sensitivity Mutants
Substantial progress towards understanding at least some developmental processes in animals has been made by working with species (model systems) that are often of no direct economic import. To date, the use o...
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Article
Purification and partial characterisation of two abscisic-acid-responsive proteins induced in cultured embryos ofPisum sativum L.
When pea (Pisum sativum L.) embryos were cultured on low osmotica, with or without added abscisic acid (ABA), there was very little change in the total mRNA translation products resolved by one-dimensional sodium...
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Article
A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves
A monoclonal antibody produced to abscisic acid (ABA) has been characterised and the development of a radioimmunoassay (RIA) for ABA using the antibody is described. The antibody had a high selectivity for the...
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Article
An analysis of seed development in Pisum sativum. VII. Embryo development and precocious germination in vitro
Three different culture media have been examined for their ability to support growth in culture of embryos of two pea lines near-isogenic except for the r-locus. Embryos showed a greater increase in fresh weight ...
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Article
Immunoassay and ultrastructural localization of isopentenyladenine and related cytokinins using monoclonal antibodies
Two hybridoma cell lines, J40-IV-A1 and J40-IV-C4 were obtained from a fusion of spleen cells of Balb/c mice immunized against an isopentenyladenosine-bovine serum albumin conjugate with X63. Ag 8.653 myeloma ...
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Article
An analysis of seed development inPisum sativum V. Fluorescence triple staining for investigating cotyledon cell development
A triple staining technique has been developed to investigate the relationship between the increase in DNA content and initiation of storage protein synthesis in pea cotyledon cells. Cells were separated by in...
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Article
Characterisation of a temperature-sensitive gametophore over-producing mutant of the moss, Physcomitrella patens
A mutant of the moss, Physcomitrella patens, was isolated which was temperature-sensitive for the production of gametophores. At 17° C this mutant, designated ove 409, produced normal leafy shoots. At 24° C ove 4...
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Chapter and Conference Paper
Immunoassays for Plant Growth Regulators — A Help or a Hindrance?
The improvements in our ability to detect and quantify endogenous plant growth regulators have reflected advances in analytical techniques to a certain extent. The literature reveals that detection and quantif...