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Article
Die operative Therapie des Vulvakarzinoms
Die Prognose des Vulvakarzinoms wird wesentlich durch die operative Therapie bestimmt. Neben der onkologischen Sicherheit müssen rekonstruktive Maßnahmen Berücksichtigung finden. Die Erkrankung tritt immer häu...
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Article
Entzündliche Erkrankungen des Uterus und der Adnexe
Entzündliche Erkrankungen von Uterus und Adnexen entstehen meist durch vaginale Keimaszension, seltener durch intraabdominale Prozesse, und werden als „pelvic inflammatory disease“ (PID) bezeichnet. Die Klinik...
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Article
Multiresistente Keime
Die Zunahme von durch multiresistente Erreger (MRE) verursachten Infektionen stellt ein mittlerweile weltweites Problem dar. Dabei ist die Entwicklung von Antibiotikaresistenzen v. a. auf übermäßigen Antibioti...
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Article
Point-of-care (POC) diagnosis of bacterial vaginosis (BV) using VGTest™ ion mobility spectrometry (IMS) in a routine ambulatory care gynecology clinic
A new CE-marked portable desktop ion mobility spectrometer (VGTest) was used for detection of malodorous biogenic amines indicative of bacterial vaginosis (BV). This study aimed to assess the performance of th...
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Article
Allogeneic gene-modified tumor cells (RCC-26/IL-7/CD80) as a vaccine in patients with metastatic renal cell cancer: a clinical phase-I study
Despite novel targeted agents, prognosis of metastatic renal cell cancer (RCC) remains poor, and experimental therapeutic strategies are warranted. Transfection of tumor cells with co-stimulatory molecules and...
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Article
The immune response to sporadic colorectal cancer in a novel mouse model
Current mouse models do not reflect the sporadic nature of colon cancer and do not allow the analysis of antitumor immune response because of the lack of known tumor-specific antigens. Two transgenic mouse mod...
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Article
In vivo splenic CD11c cells downregulate CD4 T-cell response thereby decreasing systemic immunity to gene-modified tumour cell vaccine
One of the factors influencing the efficacy of tumour cell vaccines is the site of immunization. We have shown previously that gene-modified vaccines delivered directly inside the spleen induced antigen cross-...
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Article
Adenoviral transduction of tumor cells induces apoptosis in co-cultured T lymphocytes
Adenoviral gene transfer of immunmodulatory molecules has been employed successfully in tumor vaccination studies to induce rejection of transplanted syngeneic tumors. In contrast, the response observed when t...
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Article
Expression of B7.1 (CD80) in a renal cell carcinoma line allows expansion of tumor-associated cytotoxic T lymphocytes in the presence of an alloresponse
We have selected a well-characterized human renal cell carcinoma (RCC) line as the basis for development of a genetically engineered tumor cell vaccine to be applied in an allogeneic setting. This cell line wa...
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Article
Bcr/abl+ autologous dendritic cells for vaccination in chronic myeloid leukemia
In chronic myeloid leukemia (CML) ex vivo generated DC are characterized by constitutive expression of bcr/abl and possibly other yet undefined leukemia-associated antigens, since these DC share a common progeny ...
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Article
Allogeneic vaccination for renal cell carcinoma: development and monitoring
An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shar...
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Chapter and Conference Paper
Schnelle Etablierung humaner Mammakarzinomzellinien durch SV40 large T Antigen-Immortalisierung: Ein neuer Ansatz für gentherapeutische Strategien?
Hintergrund und Fragestellung: Die Etablierung von humanen Zellinien wurde bisher häufig limitiert durch die in der Regel nach einigen Passagen auftretende Krise der Zellen in Kultur. Zur Charakt...
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Chapter
Vaccines using Gene-Modified Tumor Cells
To date, many cytokines and costimulatory molecules have been transfected in a number of different rodent tumors and in several cases, these gene modified tumor cells, when used as vaccines, induced tumor immu...
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Article
Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells
The question of whether a retroviral vector is expressed in vivo after infection of hematopoietic stem cells remains unpredictable. In this study, we show that a switch off of retroviral sequences can be teste...
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Chapter
Influence of Local Cytokines on Tumor Metastasis: Using Cytokine Gene-Transfected Tumor Cells As Experimental Models
The molecular mechanisms and, in particular, the role of cytokines involved in the process of metastasis, though one of the most important aspects of cancer research, are largely unknown. In order to metastasi...
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Article
Expression of interleukin 10 in human melanoma
The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary ...
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Chapter and Conference Paper
Risiko der Rektumchirurgie im hohen Alter
Colorectal cancer is the second frequent cancer as well in man as in woman. Expecting a steep increase of old persons in the following years the risk of colorectal cancer surgery with priority of rectal cancer...
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Chapter and Conference Paper
Cytokine Involvement in Tumor Development and Tumor Suppression
Cytokines constitute a class of soluble small proteins which previously have been designated monokines, lymphokines, or interleukins. They represent the intercellular signaling system for hematopoiesis and imm...
