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Article
A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase
The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation. Advances in glycoengineering o...
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Article
In vitro enzymatic treatment to remove O-linked mannose from intact glycoproteins
The methylotrophic yeast Pichia pastoris is an attractive expression system for heterologous protein production due to its ability to perform posttranslational modifications, such as glycosylation, and secrete la...
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Article
Elimination of diaminopeptidase activity in Pichia pastoris for therapeutic protein production
Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylati...