18 Result(s)
within
Steven J. Klosterman
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Article
Open AccessThe acyl-CoA-binding protein VdAcb1 is essential for carbon starvation response and contributes to virulence in Verticillium dahliae
In the face of carbon, nitrogen, and phosphorus starvation, microorganisms have evolved adaptive mechanisms to maintain growth. In a previous study, we identified a protein predicted to contain acyl-CoA-bindin...
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Open AccessFunctional analysis of the mating type genes in Verticillium dahliae
Populations of the plant pathogenic fungus Verticillium dahliae display a complex and rich genetic diversity, yet the existence of sexual reproduction in the fungus remains contested. As pivotal genes, MAT genes ...
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Article
Belowground microbiota associated with the progression of Verticillium wilt of smoke trees
The diversity and stability of belowground microbiota communities influence plant health. However, changes over time in the microbiome composition associated with healthy and diseased trees remains poorly unde...
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Article
Open AccessTwo zinc finger proteins, VdZFP1 and VdZFP2, interact with VdCmr1 to promote melanized microsclerotia development and stress tolerance in Verticillium dahliae
Melanin plays important roles in morphological development, survival, host–pathogen interactions and in the virulence of phytopathogenic fungi. In Verticillum dahliae, increases in melanin are recognized as marke...
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Article
Open AccessGenome-wide identification and analysis of a cotton secretome reveals its role in resistance against Verticillium dahliae
The extracellular space between the cell wall and plasma membrane is a battlefield in plant-pathogen interactions. Within this space, the pathogen employs its secretome to attack the host in a variety of ways,...
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Article
Open AccessSeptins regulate virulence in Verticillium dahliae and differentially contribute to microsclerotial formation and stress responses
Septin proteins play a role in the formation of hyphal septa as well as in the division of nuclei, cytoskeletal organization, and cell morphogenesis in filamentous fungi. Herein, we investigated the functions ...
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Open AccessA polyketide synthase from Verticillium dahliae modulates melanin biosynthesis and hyphal growth to promote virulence
During the disease cycle, plant pathogenic fungi exhibit a morphological transition between hyphal growth (the phase of active infection) and the production of long-term survival structures that remain dormant...
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Open AccessVerticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence
Verticillium dahliae is a fungal pathogen that causes a vascular wilt on many economically important crops. Common fungal extracellular membrane (CFEM) domain proteins including secreted types have been implicate...
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Article
Open AccessDynamics of Verticillium dahliae race 1 population under managed agricultural ecosystems
Plant pathogens and their hosts undergo adaptive changes in managed agricultural ecosystems, by overcoming host resistance, but the underlying genetic adaptations are difficult to determine in natural settings. V...
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Article
GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae
GhMYB4 acts as a negative regulator in lignin biosynthesis, which results in alteration of cell wall integrity and activation of cotton defense response.
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Article
Detached leaf inoculation assay for evaluating resistance to the spinach downy mildew pathogen
Downy mildew, caused by the obligate oomycete Peronospora effusa (Pfs), is the most economically important disease of spinach. In recent years, numerous new races of the pathogen have overcome the resistance used...
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Open AccessTranscriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew
Downy mildew of spinach is caused by the obligate oomycete pathogen, Peronospora effusa. The disease causes significant economic losses, especially in the organic sector of the industry where the use of synthetic...
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Article
Open AccessComparative genomics of downy mildews reveals potential adaptations to biotrophy
Spinach downy mildew caused by the oomycete Peronospora effusa is a significant burden on the expanding spinach production industry, especially for organic farms where synthetic fungicides cannot be deployed to c...
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Article
Open AccessDeep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae
Verticillium dahliae is a soil-borne fungus that causes vascular wilt diseases in a wide range of plant hosts. V. dahliae produces multicelled, melanized resting bodies, also known as microsclerotia (MS) that can...
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Article
Open AccessRNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae
The soilborne fungus, Verticillium dahliae, causes Verticillium wilt disease in plants. Verticillium wilt is difficult to control since V. dahliae is capable of persisting in the soil for 10 to 15 years as melani...
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Article
Open AccessTransposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification
Verticillium dahliae (Vd) and Verticillium albo-atrum (Va) are cosmopolitan soil fungi causing very disruptive vascular diseases on a wide range of crop plants. To date, no sexual stage has been identified in eit...
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Real-Time PCR for the Quantification of Fungi In Planta
Methods enabling quantification of fungi in planta can be useful for a variety of applications. In combination with information on plant disease severity, indirect quantification of fungi in planta offers an a...
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Article
Open AccessInsights from the genome of the biotrophic fungal plant pathogen Ustilago maydis
Ustilago maydis is an important fungal pathogen of maize, causing corn smut. It is well adapted to its host and proliferates in living plant tissue without inducing a defence response. The genome sequence of U. m...
