-
Article
Mtss1 promotes maturation and maintenance of cerebellar neurons via splice variant-specific effects
Efficient coupling of the actin cytoskeleton to the cell membrane is crucial for histogenesis and maintenance of the nervous system. At this critical interface, BAR (Bin–Amphiphysin–Rvs) proteins regulate memb...
-
Article
Open AccessThe importance of nerve microenvironment for schwannoma development
Schwannomas are predominantly benign nerve sheath neoplasms caused by Nf2 gene inactivation. Presently, treatment options are mainly limited to surgical tumor resection due to the lack of effective pharmacologica...
-
Article
Efficient and graded gene expression in glia and neurons of primary cerebellar cultures transduced by lentiviral vectors
Lentiviral vectors are valuable tools to express genes of interest in living animals and stem cell cultures. The use of promoters in lentiviral constructs has been successfully used to drive gene expression in...
-
Article
Merlin isoform 2 in neurofibromatosis type 2–associated polyneuropathy
Neurofibromatosis type 2 (NF2) is caused by inactivation of the NF2 gene, which encodes merlin. NF2 patients develop peripheral neuropathies. The authors show that NF2 inactivation decreases axonal integrity in m...
-
Article
Physiological Purkinje Cell Death Is Spatiotemporally Organized in the Develo** Mouse Cerebellum
Physiological cell death is crucial for matching defined cellular populations within the central nervous system. Whereas the time course of developmental cell death in the central nervous system is well analyz...
-
Article
Open AccessBesides Purkinje cells and granule neurons: an appraisal of the cell biology of the interneurons of the cerebellar cortex
Ever since the groundbreaking work of Ramon y Cajal, the cerebellar cortex has been recognized as one of the most regularly structured and wired parts of the brain formed by a rather limited set of distinct ce...
-
Article
Open AccessDevelopmental expression and differentiation-related neuron-specific splicing of metastasis suppressor 1(Mtss1) in normal and transformed cerebellar cells
Mtss1 encodes an actin-binding protein, dysregulated in a variety of tumors, that interacts with sonic hedgehog/Gli signaling in epidermal cells. Given the prime importance of this pathway for cerebellar devel...
-
Article
Aldolase C/Zebrin II is Released to the Extracellular Space after Stroke and Inhibits the Network Activity of Cortical Neurons
Cell death after stroke involves apoptotic, autophagocytic and necrotic mechanisms which may cause the release of cytosolic proteins to the extracellular space. Aldolase C (AldC) is the brain specific isoform ...
-
Article
High level Purkinje cell specific expression of green fluorescent protein in transgenic mice
The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells al...
-
Article
Subcellular localization of specific mRNAs and their protein products in Purkinje cells by combined fluorescence in situ hybridization and immunocytochemistry
In this study we have investigated the subcellular distribution of two mRNAs coding for the Purkinje cell-specific proteins, calbindin D28K and L7 (L7/pcp-2). Whereas calbindin mRNA was found to be in the cell b...
-
Article
Ascorbic-acid-mediated iron release from cellular ferritin and its relation to the formation of DNA strand breaks in neuroblastoma cells
Ascorbic acid at pharmacologically attainable concentrations effectively inhibited the growth of the catecholamine-positive neuroblastoma cell line SK-N-SH; it inhibited LS cells to a smaller extent and catech...
-
Article
Ferritin enhances production of DNA strand breaks by 6-hydroxydopamine, ascorbic acid and H2O2 in CCC PM2 bacteriophage DNA
Damage of CCC PM2 DNA by 6-hydroxydopamine (6-OHDA) and ascorbic acid (AA), compounds that are both able to release iron from ferritin, was significantly enhanced in the presence of ferritin. H2O2, a product of 6...
