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Article
Open AccessInter-laboratory Validation of an HPLC–MS/MS Method for the Detection of Microbial Transglutaminase in Meat and Meat Products
Microbial transglutaminase (TG) is an enzyme isolated on an industrial scale from Streptomyces mobaraensis. Technical TG, a formulated powder, is primarily used to restructure meat in the meat-processing industry...
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Article
Valid machine learning algorithms for multiparameter methods
In the light of recent food fraud cases, the issue of food authenticity is receiving increasing attention. New analytical methods and evaluation approaches are currently being proposed. In this framework, the ...
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Article
Taking laboratory uncertainties into account in the Hampel estimator
According to ISO/IEC 17025:2017, testing and calibration laboratories should apply procedures for estimating measurement uncertainty. It would thus make sense to include these uncertainty estimates in the eval...
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Article
Efficient estimation of interlaboratory and in-house reproducibility standard deviation in factorial validation studies
In case that fewer laboratories are available than required in order to ensure reliable precision estimates, the method validation study approach that is put forward in ISO 5725-2 is not applicable. Nested des...
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Article
Das erste Treffen der § 64 LFGB Arbeitsgruppe „Massenspektrometrische Proteinanalytik“
Massenspektrometrische Verfahren verfügen über ein großes Potenzial für die Bestimmung von Proteinen in Lebensmitteln. Die Kombination von Flüssigchromatografie und massenspektrometrischen Fragmentierungstechn...
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Open AccessEfficient estimation of the limit of detection and the relative limit of detection along with their reproducibility in the validation of qualitative microbiological methods by means of generalized linear mixed models
The reproducibility of measurement results is a core performance characteristic for quantitative methods. However, in the validation of qualitative methods it is not clear how to characterize a method’s reprod...
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Article
Open AccessCarcinoembryonic antigen and cytokeratin-19 fragments for assessment of therapy response in non-small cell lung cancer: a systematic review and meta-analysis
This meta-analysis evaluated whether pretherapy serum levels of carcinoembryonic antigen (CEA) and cytokeratin-19 fragments (CYFRA 21-1) are predictive of response to therapy in non-small cell lung cancer (NSC...
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Article
Robust estimation of between and within laboratory standard deviation with measurement results below the detection limit
In method validation studies, the question arises how to deal with <LOD results in order to ensure reliable precision estimates. It is proposed to elaborate strategies for dealing with <LOD results on the basis o...
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Article
Qualitative PT data analysis with easy-to-interpret scores
Numerical values called L-scores, with properties similar to those of z-scores, can be calculated for proficiency tests involving qualitative test methods. These scores do not require the use of replicates and ar...
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Article
Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater
This study describes the development of a bioassay to detect the presence of progesterone and progesterone-like molecules in wastewater samples. The basis of the bioassay is the integration of the human proges...
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Article
Validation of qualitative PCR methods on the basis of mathematical–statistical modelling of the probability of detection
A new model for the probability of detection (POD curve) for qualitative PCR methods examined in a method validation collaborative study is presented. The model allows the calculation of the POD curve and the lim...
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Article
Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products
Presence of genetic modifications in rice products originating from China and imported to the European Union market is detected since 2006. Neither these products from China nor any other genetically modified ...
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Article
A new profile likelihood confidence interval for the mean probability of detection in collaborative studies of binary test methods
A confidence interval for the probability of detection across laboratories (LPOD) for qualitative methods, described in the AOAC INTERNATIONAL guidelines for validation of microbiological methods for food and env...
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Article
Can the usual validation standard series for quantitative methods, ISO 5725, be also applied for qualitative methods?
Repeatability standard deviation, laboratory standard deviation, and reproducibility standard deviation for quantitative methods according to ISO 5725 series were recently proposed to estimate the precision of...
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Article
Results of a European interlaboratory method validation study for the quantitative determination of lipophilic marine biotoxins in raw and cooked shellfish based on high-performance liquid chromatography–tandem mass spectrometry. Part I: collaborative study
A European interlaboratory collaborative study was conducted to validate a method for the quantitative determination of lipophilic marine biotoxins based on high-performance liquid chromatography–tandem mass s...
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Article
Factorial interlaboratory tests for microbiological measurement methods
This paper presents a validation approach for microbiological methods based on a combination of interlaboratory tests and factorial experiments. It requires not more than 4 participants but is achieving compar...
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Article
Wollen Sie in Ihrem Beruf glücklich sein?
Ein Freier Träger in Ostdeutschland, vor 15 Jahren aus einer Idee heraus geboren: Rückblick auf die Entwicklung von „Rückenwind e.V. Schönebeck“, einem Stück Sozialer Arbeit im Osten.
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Reference material in residue control: assessment of matrix effects
The production of certified reference materials (CRM) requires a comprehensive consideration of which analytes and materials to select. The present study describes the pre-tests for the production of CRM for n...
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Article
Macrozoobenthos interlaboratory comparison on taxonomical identification and counting of marine invertebrates in artificial sediment samples including testing various statistical methods of data evaluation
A macrozoobenthos interlaboratory comparison was carried out by 16 laboratories to check the taxonomical expertise and the precision of sorting and counting. Participating laboratories had to determine and cou...
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Article
Chlorophyll-a determination: results of an interlaboratory comparison
A chlorophyll-a interlaboratory comparison was carried out to compare three different analytical chlorophyll-a determination methods: a German standard DIN 38412-16, a method of the HELCOM-Combine-Manual and the ...
