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Article
Effectiveness of community hospital post-acute care on mortality, re-admission, institutionalization, and activation of a home care programme in Emilia-Romagna region, Italy
In Italy, there is scant evidence on the impact of Community Hospitals (CHs) on clinical outcomes.
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Article
Open AccessDevelopment of a Quantitative UPLC-ESI/MS Method for the Simultaneous Determination of the Chitin and Protein Content in Insects
In a context where the commercial and nutritional interest in insect chitin is always increasing, an accurate and precise method to quantify this biopolymer, especially in food/feed, is required. In addition, ...
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Article
Open AccessEffectiveness of enzymatic hydrolysis for reducing the allergenic potential of legume by-products
The interest in agri-food residues and their valorization has grown considerably, and many of them are today considered to be valuable, under-exploited sources of different compounds and notably proteins. Desp...
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Chapter
Food (In)Security: The Role of Novel Foods on Sustainability
Food production today impacts heavily on the environment and available resources while at the same time failing to provide equal access to food security and healthy diets for everyone. To improve this situatio...
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Article
The effect of pre-slaughter starvation on muscle protein degradation in sea bream (Sparus aurata): formation of ACE inhibitory peptides and increased digestibility of fillet
High-quality proteins from farmed fish, including sea bream, represent a cheaper and healthier alternative to proteins from other animals, especially for people affected by chronic diseases. Aquaculture has al...
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Article
Open AccessShotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates
Since 2018, insects have belonged the category of Novel Foods and the presence of allergens represents one of the main hazards connected to their consumption, also due to the potential cross-reactivity with Ar...
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Article
Improving the Lipophilic Antioxidant Activity of Poultry Protein Hydrolisates Through Chemical Esterification
Poultry protein hydrolysates (PPHs) are obtained as by-product of poultry slaughter industry using bio catalytic hydrolysis. These protein hydrolysates have good antioxidant properties, which make them potenti...
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Article
A Complete Mass Spectrometry (MS)-Based Peptidomic Description of Gluten Peptides Generated During In Vitro Gastrointestinal Digestion of Durum Wheat: Implication for Celiac Disease
Resistance of gluten to gastrointestinal digestion is involved in immune-mediated adverse reactions to wheat, since several peptides produced by the incomplete digestion are able to trigger, in predisposed ind...
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Article
Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss)
Using Western blotting and mass spectrometry we investigated the changes in trout muscle proteins as affected by pre-slaughter nutrient supply practices, including starvation and feed restriction, in compariso...
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Article
Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor
Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics. The main aim of the present work was to investigate a new process allowi...
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Article
Degradation of Collagen Increases Nitrogen Solubilisation During Enzymatic Hydrolysis of Fleshing Meat
The meat portion directly attached to bovine hides (fleshing meat) is a by-product of leather industry that is a potential new source of proteins. In literature different enzymatic and chemical methods have be...
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Article
Enzymatic pectic oligosaccharides (POS) production from sugar beet pulp using response surface methodology
Pectic oligosaccharides (POS) have been indicated as novel candidate prebiotics. Traditionally, POS are produced from pectin-rich by-products using a two-step process involving extraction of the pectin, follow...
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Article
Proteolytic resistance of actin but not of myosin heavy chain during processing of Italian PDO (protected designation of origin) dry-cured hams
Proteomics is the approach of choice to study the fate of specific proteins, and it is very useful in identifying quality molecular markers. A combination of immunochemical and mass spectrometry analysis was u...
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Chapter
The Strategy for Screening of Antioxidant Constituents in Protein Hydrolysates
More than 100 antioxidant peptides have been identified recently in protein hydrolysates and fermented foodstuffs. Antioxidant activity of peptides is essential both for their application as functional food in...
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Article
Open AccessDiscovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase
Many agricultural and industrial food by-products are rich in cellulose and xylan. Their enzymatic degradation into monosaccharides is seen as a basis for the production of biofuels and bio-based chemicals. Ly...
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Article
Introducing enzyme selectivity: a quantitative parameter to describe enzymatic protein hydrolysis
Enzyme selectivity is introduced as a quantitative parameter to describe the rate at which individual cleavage sites in a protein substrate are hydrolyzed relative to other cleavage sites. Whey protein isolate...
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Article
Qualitative and quantitative determination of peptides related to celiac disease in mixtures derived from different methods of simulated gastrointestinal digestion of wheat products
During wheat digestion, gluten-derived proteolytic resistant peptides are generated, some of them involved in celiac disease. In vitro digestion models able to mimic the peptides generated in the human gastroi...
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Article
Enzymatic production and degradation of cheese-derived non-proteolytic aminoacyl derivatives
Gamma-glutamyl-amino acids, lactoyl-amino acids and pyroglutamyl-amino acids, collectively named Non-Proteolytic Aminoacyl Derivatives (NPADs) are unusual aminoacyl derivatives of non-proteolytic origins found...
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Protocol
Use of Peptide Nucleic Acids (PNAs) for Genoty** by Solution and Surface Methods
Peptide nucleic acids (PNAs) are synthetic oligonucleotide analogues based on a pseudopeptide backbone that bind complementary DNA or RNA with high affinity and specificity. In this chapter, three PNA-based ge...
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Protocol
Chiral PNAs with Constrained Open-Chain Backbones
Chiral open-chain PNAs have been shown to have improved properties in terms of control of helical handedness, DNA affinity, sequence selectivity, and cellular uptake. They can be synthesized either using prefo...
