46 Result(s)
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Simranjeet Singh Sekhon
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Article
Open AccessStructure based innovative approach to analyze aptaprobe–GPC3 complexes in hepatocellular carcinoma
Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is a biomarker of hepatocellular carcinoma (HCC) progression. Aptamers specifically binding to target biomolecules have recently emerged as cli...
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Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency
mRNA vaccines hold great potential as therapeutic techniques against viral infections due to their efficacy, safety, and large-scale production. mRNA vaccines offer flexibility in development as any protein ca...
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Codon optimization of the synthetic 3-ketosphinganine reductase (3KSR) protein for enhancing sphingolipid biosynthetic enzyme expression
Sphinganine is a key precursor for the synthesis of ceramide during sphingolipid biosynthesis in eukaryotes. In the sphinganine biosynthetic pathway, serine palmitoyltransferase (SPT) and 3-ketosphinganine red...
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Heterologous expression of a novel serine palmitoyltransferase from Sphingobium chungbukense
In the described experiments, we identify and clone a novel serine palmitoyltransferase (SPT) isolated from the gram-negative obligatory aerobic bacteria Sphingobium chungbukense strain KCTC2955. SPT catalyzes th...
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Article
Effects of Light Regulation on Proteome Expression in Rhodobacter sphaeroides 2.4.1
Light plays an important role in the transcriptional regulation of photosynthetic apparatus. The influence of oxygen and light conditions on the protein expression of Rhodobacter sphaeroides was investigated usin...
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Article
Open Access2D graphene oxide–aptamer conjugate materials for cancer diagnosis
2D graphene oxide (GO) with large surface area, multivalent structure can easily bind single-stranded DNA/RNA (aptamers) through hydrophobic/π-stacking interactions, whereas aptamers having small size, excelle...
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Article
The pine wood nematode Bursaphelenchus xylophilus and molecular diagnostic methods
Bursaphelenchus xylophilus, commonly known as the pine wood nematode (PWN), is one of the most destructive nematode species. It causes pine wilt, a slow progressive disease that results in economic loss worldwide...
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Aptamer-linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)
Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM cro...
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The Role of Aptamer Loaded Exosome Complexes in the Neurodegenerative Diseases
Extracellular exosomes formed inside the cytoplasm of cells are small membranous vesicles that provide an important mode of cell-to-cell communication by delivering proteins, lipids and RNA to target cells. Ex...
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Aptasensors for Pesticide Detection
Pesticides are one of the prominent issues in food safety and environmental pollution, and a rapid pesticide residue detection method is urgently needed to keep humans from being affected. Aptamers are single ...
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Article
Bioengineered aptamer-nanoconjugates for cancer theragnosis
Early detection and treatment of cancer is of global interest as cancer is one of the leading causes of death worldwide. As the survival rate of cancer patients is closely related to its detection at an early ...
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Article
Lactic Acid Bacteria Isolated from Kimchi to Evaluate Anti-obesity Effect in High Fat Diet-induced Obese Mice
Obesity is one of the major public concerns due to its various side-effects such as metabolic and chronic ailments. In this study, two lactic acid bacteria (LAB) strains, Leuconostoc mesenteroides and Lactobac...
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Anti-inflammatory effect of lactic acid bacteria isolated from kimchi on acid-induced acute colitis in model mice
Lactic acid bacteria (LAB) provide numerous beneficial effects on the host body, especially on the intestine. Two LAB strains isolated from Kimchi, Leuconostoc mesenteroides and Lactobacillus sakei, were studi...
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Detection of endotoxins using nanomaterials
An endotoxin is a lipopolysaccharide complex that is associated with the outer membrane of gram negative bacteria, and even a small amount can trigger strong exothermic activity. Pharmaceutical and food produc...
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Effect of heavy metals, pesticides and pharmaceuticals on yeast’s vacuoles as a biomarker for toxic detection
In this study, the effect of different toxic chemicals on Saccharomyces cerevisiae’s vacuoles was determined. More precisely, oxidative stress was mediated by pesticides and heavy metals, whereas nonoxidative str...
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Aptasensor for environmental monitoring
Aptamers are single-stranded oligonucleotides (DNA or RNA) capable of folding into well-defined structures and motifs that allow them bind to various target molecules with high specificity and affinity. The cr...
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Article
The effect of phosphodiesterase (PDEs) 4C to regulation the activity of lysosomes
Phosphodiesterases (PDEs) are involved in many cellular signal transductions mediated by cAMP. The PDEs are involved in phosphorylation when the precursors of soluble lysosomal enzymes transport into the lysos...
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Article
Porcine epidemic diarrhea (PED) infection, diagnosis and vaccination: A mini review
Porcine epidemic diarrhea virus (PEDV) is a main etiology causing severe enteric disease in piglets with clinical signs of anorexia, vomiting, diarrhea and dehydration resulting in loss of condition and death ...
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Article
Enhanced lysosomal activity by overexpressed aminopeptidase Y in Saccharomyces cerevisiae
Saccharomyces cerevisiae contains vacuoles corresponding to lysosomes in higher eukaryotes. Lysosomes are dynamic (not silent) organelles in which enzymes can be easily integrated or rele...
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Lysosome-related organelles extracts from egg-white for hypermelanosis reduction
Pigmentary disorders generally indicate that an increased amount of melanin, leading to darker color of skin, called hypermelanosis. Thus, several methods were studied for finding therapy of hypermelanosis unt...
