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Article
Open AccessWhole genome sequencing of mouse lines divergently selected for fatness (FLI) and leanness (FHI) revealed several genetic variants as candidates for novel obesity genes
Analysing genomes of animal model organisms is widely used for understanding the genetic basis of complex traits and diseases, such as obesity, for which only a few mouse models exist, however, without their l...
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Article
Open AccessGenome-wide screening for genetic variants in polyadenylation signal (PAS) sites in mouse selection lines for fatness and leanness
Alternative polyadenylation (APA) determines mRNA stability, localisation, translation and protein function. Several diseases, including obesity, have been linked to APA. Studies have shown that single nucleot...
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Chapter and Conference Paper
Analysis of Slovak Court Decisions by Formal Concept Analysis and Machine Learning Methods
The applications of machine learning and artificial intelligence methods in the natural language processing of court decisions have become an important research topic worldwide. In this paper, we review our da...
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Article
Genetic variants of the hypoxia‐inducible factor 3 alpha subunit (Hif3a) gene in the Fat and Lean mouse selection lines
Adipose tissue hypoxia and members of the hypoxia-inducible factor alpha (HIFA) are involved in development of obesity. However, the mechanism and functions of HIF3A, one of three HIFA paralogs, in fat deposit...
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Chapter and Conference Paper
Cordelia: An Application for Automatic ECG Diagnostics
The authors present a prototype of an application named Cordelia, which enables the prediction of selected cardiac findings on standard 12-lead ECG recordings. The application is based on an ensemble model con...
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Article
Open AccessPost-genoty** optimization of dataset formation could affect genetic diversity parameters: an example of analyses with alpine goat breeds
Local breeds retained unique genetic variability important for adaptive potential especially in light of challenges related to climate change. Our first objective was to perform, for the first time, a genome-w...
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Article
Open AccessNeurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice
Long-acting local anaesthetics (e.g. bupivacaine hydrochloride) or sustained-release formulations of bupivacaine (e.g. liposomal bupivacaine) may be neurotoxic when applied in the setting of diabetic neuropath...
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Article
Open AccessCRISPRa-mediated FOXP3 gene upregulation in mammalian cells
Forkhead box P3+ (FOXP3+) regulatory T cells (Tregs) are a subset of lymphocytes, critical for the maintenance of immune homeostasis. Loss-of-function mutations of the FOXP3 gene in animal models and humans resul...
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Article
3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice
In obesity, the skeletal muscle capillary network regresses and the insulin-mediated capillary recruitment is impaired. However, it has been shown that in the early stage of advanced obesity, an increased func...
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Article
Correction: Corrigendum: Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness
Nat. Med. 22, 771–779 (2016); published online 6 June 2016; corrected after print 7 March 2018 In the version of this article initially published, the colors of the lines were switched in the graph that shows ...
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Article
Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness
Genetic analysis in mice selected for leanness has identified thiosulfate sulfurtransferase as a target for the treatment of diabetes.
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Article
Open AccessConstruction of an integrative regulatory element and variation map of the murine Tst locus
Given the abundance of new genomic projects and gene annotations, researchers trying to pinpoint causal genetic variants are faced with a challenging task of how to efficiently integrate all current genomic in...
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Article
Open AccessLessons from Hepatocyte-Specific Cyp51 Knockout Mice: Impaired Cholesterol Synthesis Leads to Oval Cell-Driven Liver Injury
We demonstrate unequivocally that defective cholesterol synthesis is an independent determinant of liver inflammation and fibrosis. We prepared a mouse hepatocyte-specific knockout (LKO) of lanosterol 14α-deme...
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Article
Open AccessObesity resistant mechanisms in the Lean polygenic mouse model as indicated by liver transcriptome and expression of selected genes in skeletal muscle
Divergently selected Lean and Fat mouse lines represent unique models for a polygenic form of resistance and susceptibility to obesity development. Previous research on these lines focused mainly on obesity-su...
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Article
Congenic and bioinformatics analyses resolved a major-effect Fob3b QTL on mouse Chr 15 into two closely linked loci
We previously identified a Chr 15 quantitative trait locus (QTL) Fob3b in lines of mice selected for high (Fat line) and low (Lean line) body fat content that represent a unique model of polygenic obesity. Here w...
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Article
Divergent Physical Activity and Novel Alternative Responses to High Fat Feeding in Polygenic Fat and Lean Mice
We determined whether altered physical activity levels might underlie the contrasting adiposity of a divergently selected polygenic murine model of metabolic syndrome (Fat; F) and leanness (Lean; L) mice. We m...
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Article
Genetic complexity of an obesity QTL (Fob3) revealedby detailed genetic map**
Obesity is proving to be a serious health concern in the developed world as well as an unwanted component of growth in livestock production. While recent advances in genetics have identified a number of monoge...
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Article
Quantitative trait loci affecting growth in high growth (hg) mice
A genome-wide scan was performed in order to identify Quantitative Trait Loci (QTL) associated with growth in a population segregating high growth (hg), a partially recessive mutation that enhances growth rate a...
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Article
Map** the RAIDD gene of chicken (Gallus gallus): identification of a region homologous to the mouse high-growth region
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Article
Molecular cloning and partial characterisation of the mouse Cyp51 cDNA
Northern analysis of the mouse testis and liver RNA show two types of lanosterol 14α-demethylase (CYP51) transcripts, somatic and a testis-specific transcript. At least two CYP51 mRNAs are expressed in liver a...
