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Protocol
Acetylcholinesterase Inhibition Assays for High-Throughput Screening
Acetylcholinesterase (AChE) hydrolyzes acetylcholine (ACh), a vital neurotransmitter that regulates muscle movement and brain function, including memory, attention, and learning. Inhibition of AChE activity ca...
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Protocol
Cell-Based Imaging Assay for Detection of Phospholipidosis
Accumulation of lysosomal phospholipids in cells exposed to cationic amphiphilic drugs is characteristic of drug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of un...
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Chapter
High-Throughput Screening and Hazard Testing Prioritization
Tox21 is a collaborative effort among the National Center for Advancing Translational Sciences, the Environmental Protection Agency, the National Toxicology Program, and the Food and Drug Administration to elu...
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Article
Review of high-content screening applications in toxicology
High-content screening (HCS) technology combining automated microscopy and quantitative image analysis can address biological questions in academia and the pharmaceutical industry. Various HCS experimental app...
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Article
Why are most phospholipidosis inducers also hERG blockers?
Recent reports have noted that a number of compounds that block the human Ether-à-go-go related gene (hERG) ion channel also induce phospholipidosis (PLD). To explore a hypothesis explaining why most PLD induc...
