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Article
SfuPKS2, a novel type III polyketide synthase from Sargassum fusiforme, shows high substrate specificity
A type III polyketide synthase (SfuPKS2) from the edible seaweed Sargassum fusiforme was cloned and biochemically characterized. SfuPKS2 can only accept octanoyl-CoA (C8) and neither other long-chain fatty acyl-C...
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Article
Open AccessPrenylation of dimeric cyclo-l-Trp-l-Trp by the promiscuous cyclo-l-Trp-l-Ala prenyltransferase EchPT1
Prenyltransferases (PTs) from the dimethylallyl tryptophan synthase (DMATS) superfamily are known as efficient biocatalysts and mainly catalyze regioselective Friedel-Crafts alkylation of tryptophan and trypto...
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Article
Open AccessA basidomycetous hydroxynaphthalene-prenylating enzyme exhibits promiscuity toward prenyl donors
The fungal prenyltransferase ShPT from Stereum hirsutum was believed to prenylate 4-hydroxybenzyl alcohol and thereby be involved in the vibralactone biosynthesis. In this study, we demonstrate that hydroxynaphth...
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Article
Open AccessDiprenylated cyclodipeptide production by changing the prenylation sequence of the nature’s synthetic machinery
Ascomycetous fungi are often found in agricultural products and foods as contaminants. They produce hazardous mycotoxins for human and animals. On the other hand, the fungal metabolites including mycotoxins ar...
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Article
Open AccessA fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin
Biosynthesis of the flavonoid naringenin in plants and bacteria is commonly catalysed by a type III polyketide synthase (PKS) using one p-coumaroyl-CoA and three malonyl-CoA molecules as substrates. Here, we repo...
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Article
Open AccessConversion of viridicatic acid to crustosic acid by cytochrome P450 enzyme-catalysed hydroxylation and spontaneous cyclisation
Cytochrome P450 monooxygenases (P450s) are considered nature’s most versatile catalysts and play a crucial role in regio- and stereoselective oxidation reactions on a broad range of organic molecules. The oxyf...
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Article
Open AccessEffectiveness and safety of acupotomy for knee osteoarthritis: study protocol for a randomized controlled trial
Knee osteoarthritis (KOA) is one of the most common musculoskeletal disorders. Acupotomy may be effective for KOA, but the evidence is limited. This trial aims to determine the effectiveness and safety of acup...
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Article
Open AccessBenzoyl ester formation in Aspergillus ustus by hijacking the polyketide acyl intermediates with alcohols
Accumulation of two benzoyl esters in Aspergillus ustus after feeding with alcohols was reported 30 years ago. To the best of our knowledge, the biosynthesis for these esters has not been elucidated prior to this...
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Article
Open AccessModifications of diketopiperazines assembled by cyclodipeptide synthases with cytochrome P450 enzymes
2,5-Diketopiperazines are the smallest cyclic peptides comprising two amino acids connected via two peptide bonds. They can be biosynthesized in nature by two different enzyme families, either by nonribosomal pep...
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Article
Open AccessOxepinamide F biosynthesis involves enzymatic d-aminoacyl epimerization, 3H-oxepin formation, and hydroxylation induced double bond migration
Oxepinamides are derivatives of anthranilyl-containing tripeptides and share an oxepin ring and a fused pyrimidinone moiety. To the best of our knowledge, no studies have been reported on the elucidation of an...
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Article
Reinvestigation of the substrate specificity of a reverse prenyltransferase NotF from Aspergillus sp. MF297-2
NotF from Aspergillus sp. MF297-2 and BrePT from Aspergillus versicolor catalyze a reverse C2-prenylation of brevianamide F in the biosynthetic pathway of brevianamides and notoamides. NotF was reported to use on...
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Article
Open AccessComparative studies on similarities and differences of cyclodipeptide oxidases for installation of C–C double bonds at the diketopiperazine ring
Cyclodipeptide oxidases (CDOs) perform dehydrogenations on diketopiperazines and play an important role in the cyclodipeptide diversification. In this study, we investigated the two known CDOs AlbA/B and Ndas_...
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Article
Different behaviors of cyclic dipeptide prenyltransferases toward the tripeptide derivative ardeemin fumiquinazoline and its enantiomer
In nature, cyclic dipeptide prenyltransferases catalyze regioselective Friedel-Crafts alkylations of tryptophan-containing cyclic dipeptides. This enzyme class, belonging to the dimethylallyl tryptophan syntha...
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Article
Open AccessMolecular evolution of influenza B virus during 2011–2017 in Chaoyang, Bei**g, suggesting the free influenza vaccine policy
Two influenza B virus lineages, B/Victoria and B/Yamagata, are co-circulating in human population. While the two lineages are serologically distinct and TIV only contain one lineage. It is important to investi...
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Article
Preparation and Chromatographic Features of Fibrous Core–Shell HPLC Packing Material
In this study, fibrous core–shell silica particles were successfully synthesized via a one-step oil–water biphase stratification coating strategy. The core–shell silica particles were composed of 3-µm non-pore...
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Article
Correction to: Expanding tryptophan-containing cyclodipeptide synthase spectrum by identification of nine members from Streptomyces strains
The original version of this article contained mistake. After careful re-examination of the LC-MS data, the products of CDPSs “WP_031028810” and “BAU83478” should be cFL instead of cPY. We apologize for any in...
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Article
Expanding tryptophan-containing cyclodipeptide synthase spectrum by identification of nine members from Streptomyces strains
Cyclodipeptide synthases (CDPSs) comprise normally 200–300 amino acid residues and are mainly found in bacteria. They hijack aminoacyl-tRNAs from the ribosomal machinery for cyclodipeptide formation. In this s...
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Article
Convenient synthetic approach for tri- and tetraprenylated cyclodipeptides by consecutive enzymatic prenylations
The prenyltransferases EchPT1 and EchPT2 from Aspergillus ruber are responsible for the consecutive prenylations of cyclo-l-Trp-l-Ala, leading to the formation of the triprenylated echinulin as the predominant pr...
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Article
Production of α-keto carboxylic acid dimers in yeast by overexpression of NRPS-like genes from Aspergillus terreus
Non-ribosomal peptide synthetases (NRPSs) are key enzymes in microorganisms for the assembly of peptide backbones of biologically and pharmacologically active natural products. The monomodular NRPS-like enzyme...
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Article
Mutation on Gly115 and Tyr205 of the cyclic dipeptide C2-prenyltransferase FtmPT1 increases its catalytic activity toward hydroxynaphthalenes
The fungal cyclic dipeptide prenyltransferase FtmPT1 from Aspergillus fumigatus catalyzes a regular C2-prenylation of brevianamide F (cyclo-l-Trp-l-Pro) and is involved in the biosynthesis of a number of biologic...
