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Article
Localization and biodistribution of conjugate ATG-Dex-DNR in nude mice as models for human leukemia
131I-labelled anti-thymoglobuline (ATG),131I-labelled immunoconjugate ATG-Dex-DNR and131 I-labelled Ts-MoAb as control antibody, respectively, were injected by intraperitoneal (i. p.) administrat...
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Article
Study on lymphocyte activation and proliferation induced by anti-CD3 McAb
T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro. Several interfering factors were also discussed. The result indicated that lymphocyte activation and...
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Article
Effects of cimetidine on IL-2 and T suppressor cell function in rats with obstructive jaundice
Susceptibility to infection in patients with obstructive jaundice is much more higher than non-jaundiced patients. The reasons for this are not completely understood. It is postulated that this may have some r...
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Article
Experimental study on anti-tumor effect of splenocytes induced by anti-CD3 McAb, PHA and IL-2
The proliferation of splenocytes from healthy adults was induced by anti-CD3 McAb, PHA and IL-2. The proliferative capability and anti-tumor activity as well as phenotypes of the splenocytes cultured in differ...
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Article
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: VI. Purification and relative affinity of monoclonal antibodies
In this study, the anti-idiotypic and anti-isotypic antibodies (McAbs) against IgM of the Patient with B chronic lymphocytic leukemia (B-CLL) were purified from hybridoma ascites by n-Octoic acid precipitation...
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Article
Determination of IL-2 and cytotoxicity of killer cells in MTT colorimetry
The activity of interleukine 2 (IL-2) in culture supernatants of lymphokine-activated killer (LAK) cells and tumor infiltrating lymphocytes (TIL) as well as cytotoxicity of LAK cells on cultured leukemic cells...
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Article
An analysis of antigenic polypeptides of dengue viruses and their specificity with western blotting
Dengue virus(DV) samples which harvested after having been either propagated in C6/36 cells or passaged by murine intracerebral inoculation were investigated by Western bloting. Two different denaturing methods w...
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Article
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: V. The effects of monoclonal antibodies and interferon on the levels of cyclic nucleotides in leukemic cell lines
After the leukemic cell lines were treated with monoclonal antibodies (McAbs) and interferon (IFN-α), the changes of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in th...
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Article
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs
In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the...
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Article
Purification and immunoreactivity of monoclonal antibodies against myeloma-λ chain
In this study, two murine IgM-monoclonal antibodies (IgM-McAbs) against λ chain of myeloma protein from hybridoma ascites were purified by Sephadex G200 chromatography. The eluate of the first peak on absorpti...
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Article
Studie über ein Doppelmarkierungsverfahren zur Bestimmung von T-Lymphozytensubpopulationen
Um die Zelloberflächenmarker der peripheren Lymphozytensubpopulationen zu bestimmen, wurde ein Fluoreszenz-Rosetten-Doppelmarkierungsverfahren entwickelt. Dabei wurden gleichzeitig deutlich auf einer Zelle mit...
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Article
Studies on monoclonal anti-idiotypic and anti-isotypic antibodies against leukemia and myeloma: III. Analysis of monoclonal antibodies recognizing the antigenic epitopes by use of ELISA additivity test and microcomputer grou** programme
ELISA double antibodies additivity test was employed to identify the epitopes which can be recognized by monoclonal antibodies (McAbs) against IgM of a patient with B chronic lymphocytic leukemia (B-CLL). The ...
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Article
Preparation of AHTG-DNR conjugates and their antitumor effect in vitro
100%, 75%, 50%, 25% and 12.5% oxidized dextran T10 (Dex T1o) were used as intermediate carriers for conjugating drug daunorubicin (DNR) and antibody anti-human thymocitic globulin (AHTG), to form different imm...
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Article
Immunomodulation of musk-moxa-string therapy in patients with scrofula
The effects of musk-moxa-string therapy on the immune system in man were investigated in 39 patients with scrofula. Before treatment, the numbers of peripheral blood (PB) CD3+ and DC4+ cells and the ratio CD4+/ C...
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Article
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: I. preparation and characteristics of monoclonal anti-isotypic antibodies to igm from B chronic lymphocytic leukemia
Five hybridomas producing McAbs against human serum IgM-isotype were obtained by fusing the myeloma NS-1 cell and the RALB/C murine spleen cell which had been immunized by serum IgM of patient with B chronic l...
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Article
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: I. study on monoclonal anti-idiotypic antibodies against λ chain protein of myeloma
We used A chain extracted from urine of patient with myeloma (IgD λ) as antigen for immunizing BALB/C mice, and 86 hybridoma cell clones secreting monoclonal antibodies (McAb) were obtained after fusing twice ...
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Article
Peripheral blood and bone marrow lymphocyte subsets in patients with multiple myeloma
A panel of monoclonal antibodies (McAb) from the Wu series was used to characterize lymphocyte subsets of peripheral blood (PB) from 9 patients with multiple myeloma (MM) by means of indirect immunofluorescenc...
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Article
Studies on monoclonal anti-idiotypic antibodies to human B cell leukemia
Murine immunocytoma cell line, NS-1, was fused with spleen cells of Balb/C mice which had been stimulated by tolerogenic disaggregated human gamma globulin and immunized by purified serum IgM from the patient ...
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Article
Studies on biological characteristics and modulating factors of long-term cultured bone marrow cells of a myeloma patient in vitro
In the present study we cultured in vitro bone marrow cells from a patient with multiple myeloma (IgD) and researched into the modulation of supernatant media of various leukemic lines on the growth of culture...
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Article
Beziehung zwischen serologischen und elektronenmikroskopischen Befunden bei der Virushepatitis
Dane-Partikeln wurden in 23 HBsAg-positiven Serumproben nach Ultrazentrifugation in Sucrosegradienten in Abhängigkeit vom HBsAg-Profil der Sucrosefraktion untersucht. Dane-Partikeln fanden sich hauptsächlich i...
