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Article
Inhibiting fatty acid synthesis overcomes colistin resistance
Treating multidrug-resistant infections has increasingly relied on last-resort antibiotics, including polymyxins, for example colistin. As polymyxins are given routinely, the prevalence of their resistance is ...
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Article
A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library
Temporally resolved assays of bacterial gene expression using printed fluorescence imaging boxes (PFIboxes) are non-destructive, inexpensive and simple to prepare. Herein, we describe a full experimental pipel...
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Article
A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria
Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity—an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant ...
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Article
Open AccessA macrophage-based screen identifies antibacterial compounds selective for intracellular Salmonella Typhimurium
Salmonella Typhimurium (S. Tm) establishes systemic infection in susceptible hosts by evading the innate immune response and replicating within host phagocytes. Here, we sought to identify inhibitors of intracell...
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Article
Pentamidine sensitizes Gram-negative pathogens to antibiotics and overcomes acquired colistin resistance
The increasing use of polymyxins1 in addition to the dissemination of plasmid-borne colistin resistance threatens to cause a serious breach in our last line of defence against multidrug-resistant Gram-negative pa...
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Article
Assembly and clustering of natural antibiotics guides target identification
A retrobiosynthetic algorithm that relates known antibiotics by the similarities of their biosynthetic pathways to cluster them into distinct classes. Focusing on the telomycins helps to define the mechanism o...
