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Article
Open AccessTargeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody
We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). We show that a conformation-specifi...
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Article
A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria
Artemisinins are key anti-malarial drugs, but artemisinin resistance has been increasing; this study identifies the molecular target of artemisinins as phosphatidylinositol-3-kinase and increase of the lipid p...
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Article
Open AccessEngineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling
Many receptors function by binding to multiple ligands, each eliciting a distinct biological output. The extracellular domain of the human prolactin receptor (hPRL-R) uses an identical epitope to bind to both ...
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Article
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins
Allosteric effectors have the ability to modulate protein-ligand interactions in a controlled fashion. Now a novel class of antibody-like affinity reagents, synthetic antigen binders or sABs, are generated in vit...
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Article
Control of a biomolecular motor-powered nanodevice with an engineered chemical switch
The biophysical and biochemical properties of motor proteins have been well-studied, but these motors also show promise as mechanical components in hybrid nano-engineered systems1,2,3,4. The cytoplasmic F1 fragme...
