12 Result(s)
within
Seth Blackshaw
Life Sciences
-
Article
Open AccessGene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons
GABAergic neurons of the hypothalamus regulate many innate behaviors, but little is known about the mechanisms that control their development. We previously identified hypothalamic neurons that express the LIM...
-
Article
Open AccessEpigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin
Retinal neurogenesis occurs through partially overlap** temporal windows, driven by concerted actions of transcription factors which, in turn, may contribute to the establishment of divergent genetic program...
-
Article
A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors
A collection of 1,406 high-quality, immunoprecipitation- and immunoblotting-grade monoclonal antibodies to 737 human transcription factors is made available as a community resource, along with all validation d...
-
Article
Open AccessAssessing the model transferability for prediction of transcription factor binding sites based on chromatin accessibility
Computational prediction of transcription factor (TF) binding sites in different cell types is challenging. Recent technology development allows us to determine the genome-wide chromatin accessibility in vario...
-
Article
The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox
-
Protocol
Identification of SUMO E3 Ligase-Specific Substrates Using the HuProt Human Proteome Microarray
The functional protein microarray is a powerful and versatile systems biology and proteomics tool that allows the rapid activity profiling of thousands of proteins in parallel. We have recently developed a hum...
-
Article
Yeast one-hybrid assays for gene-centered human gene regulatory network map**
A sequence-verified collection of human transcription factors is reported. The authors used it in the enhanced yeast-one hybrid (eY1H) assay to map human gene regulatory networks. Also in this issue, Reece-Hoyes
-
Article
Systematic characterization of protein-DNA interactions
Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advance...
-
Article
Open AccessThe long noncoding RNA RNCR2 directs mouse retinal cell specification
Recent work has identified that many long mRNA-like noncoding RNAs (lncRNAs) are expressed in the develo** nervous system. Despite their abundance, the function of these ncRNAs has remained largely unexplore...
-
Article
Clustering analysis of SAGE data using a Poisson approach
Serial analysis of gene expression (SAGE) data have been poorly exploited by clustering analysis owing to the lack of appropriate statistical methods that consider their specific properties. We modeled SAGE da...
-
Article
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues
Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.
-
Chapter and Conference Paper
Molecular Mechanisms of Taste Receptor Cell Signal Transduction
Some mechanisms by which taste signals are transduced have been identified [1]. Acids appear to mediate sour taste by blocking voltage-dependent K+ channels localized on the apical surface of taste cells [2,3], a...
