20 Result(s)
within
Sergi Garcia-Manyes
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Article
Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales
The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling used by cells to sense and adapt to extracellular and intracellular mechanical forces. However, ...
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Article
Open AccessStructural anisotropy results in mechano-directional transport of proteins across nuclear pores
The nuclear pore complex regulates nucleocytoplasmic transport by means of a tightly synchronized suite of biochemical reactions. The physicochemical properties of the translocating cargos are emerging as mast...
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Protocol
Unfolding and Refolding Proteins Using Single-Molecule AFM
Single-molecule atomic force microscopy (AFM) allows capturing the conformational dynamics of an individual molecule under force. In this chapter, we describe a protocol for conducting a protein nanomechanical...
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Article
Open AccessEnzyme-less nanopore detection of post-translational modifications within long polypeptides
Means to analyse cellular proteins and their millions of variants at the single-molecule level would uncover substantial information previously unknown to biology. Nanopore technology, which underpins long-rea...
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Article
Open AccessMyc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation
Dedifferentiation is the process by which terminally differentiated cells acquire the properties of stem cells. During mouse skin wound healing, the differentiated Gata6-lineage positive cells of the sebaceous...
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Article
Open AccessAllosteric activation of vinculin by talin
The talin-vinculin axis is a key mechanosensing component of cellular focal adhesions. How talin and vinculin respond to forces and regulate one another remains unclear. By combining single-molecule magnetic t...
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Article
The role of single-protein elasticity in mechanobiology
In addition to biochemical signals and genetic considerations, mechanical forces are rapidly emerging as a master regulator of human physiology. However, the molecular mechanisms that regulate force-induced fu...
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Article
Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape
Statistical mechanics can describe the major conformational ensembles determining the equilibrium free-energy landscape of a folding protein. The challenge is to capture the full repertoire of low-occurrence c...
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Article
The mechanical stability of proteins regulates their translocation rate into the cell nucleus
A cell’s ability to react to mechanical stimuli is known to be affected by the transport of transcription factors, the proteins responsible for regulating transcription of DNA into RNA, across the membrane env...
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Article
Open AccessForcing the reversibility of a mechanochemical reaction
Mechanical force modifies the free-energy surface of chemical reactions, often enabling thermodynamically unfavoured reaction pathways. Most of our molecular understanding of force-induced reactivity is restri...
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Article
Steering chemical reactions with force
Chemical reactivity underlies our fundamental understanding of many physical and biological phenomena. Chemical reactions are typically initiated by heat, electric current or light. Albeit far less studied, me...
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Article
Open AccessTailoring protein nanomechanics with chemical reactivity
The nanomechanical properties of elastomeric proteins determine the elasticity of a variety of tissues. A widespread natural tactic to regulate protein extensibility lies in the presence of covalent disulfide ...
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Article
Open AccessProtein S-sulfenylation is a fleeting molecular switch that regulates non-enzymatic oxidative folding
The post-translational modification S-sulfenylation functions as a key sensor of oxidative stress. Yet the dynamics of sulfenic acid in proteins remains largely elusive due to its fleeting nature. Here we use ...
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Article
Open AccessThe mechanochemistry of copper reports on the directionality of unfolding in model cupredoxin proteins
Understanding the directionality and sequence of protein unfolding is crucial to elucidate the underlying folding free energy landscape. An extra layer of complexity is added in metalloproteins, where a metal ...
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Article
Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy
Here we describe a protocol for using force-clamp spectroscopy to precisely quantify the effect of force on biochemical reactions. A calibrated force is used to control the exposure of reactive sites in a sing...
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Article
Single-molecule paleoenzymology probes the chemistry of resurrected enzymes
Using phylogenetic analysis, ancestral forms of thioredoxin reflecting the sequences likely to have existed at key evolutionary points have now been synthesized and analyzed. Single-molecule analysis indicates...
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Article
Caught in the act
The dynamics of a complex chemical reaction that occurs through a well-defined intermediate have been followed at the single-molecule level using a 'nanoreactor' set-up, revealing a primary hydrogen isotope ef...
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Chapter
Force-Clamp Spectroscopy of Single Proteins
Force-clamp AFM, with its remarkable ability to manipulate short recombinant proteins, has become a useful probe of protein dynamics, allowing us to sense conformational changes down to the sub-Ångström scale....
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Article
Force-activated reactivity switch in a bimolecular chemical reaction
The effect of mechanical force on the free-energy surface that governs a chemical reaction is largely unknown. The combination of protein engineering with single-molecule force-clamp spectroscopy allows us to ...
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Article
Nanoindentation: From forces to energies
We have performed nanoindentations using the tip of an AFM cantilever. Experiments have been made on a freshly etched hydrogen terminated Silicon (111) surface, yielding penetrations of less than 1 nm so that ...
