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Article
An armed oncolytic virus for GBM destruction
Immunovirotherapy is a promising therapeutic strategy for glioblastoma (GBM), a deadly tumor for which effective treatments remain a clinical need. A new study describes an oncolytic herpes simplex virus (oHSV...
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Article
Open AccessCharacterization and oncolytic virus targeting of FAP-expressing tumor-associated pericytes in glioblastoma
Cancer-associated fibroblasts (CAFs) are activated fibroblasts constituting the major stromal components in many types of cancer. CAFs contribute to hallmarks of cancer such as proliferation, invasion and immu...
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Protocol
Immunohistochemistry for Tumor-Infiltrating Immune Cells After Oncolytic Virotherapy
Immunohistochemistry (IHC) is an integral laboratory staining technique, which is used for the detection of immune cells in mouse/human tissues or tumors. Oncolytic herpes simplex virus (oHSV) treatment or vir...
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Article
A Monoclonal Antibody Against β1 Integrin Inhibits Proliferation and Increases Survival in an Orthotopic Model of High-Grade Meningioma
High-grade meningiomas (HGMs; World Health Organization [WHO] classification grade II and III) have high relapse rates and poor clinical outcomes despite surgery and radiation treatments. No effective medical ...
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Article
Open AccessMyc targeted CDK18 promotes ATR and homologous recombination to mediate PARP inhibitor resistance in glioblastoma
PARP inhibitors (PARPis) have clinical efficacy in BRCA-deficient cancers, but not BRCA-intact tumors, including glioblastoma (GBM). We show that MYC or MYCN amplification in patient-derived glioblastoma stem-lik...
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Article
Open AccessA high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS)
Cell state-specific promoters constitute essential tools for basic research and biotechnology because they activate gene expression only under certain biological conditions. Synthetic Promoters with Enhanced C...
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Article
Open AccessGenetically distinct glioma stem-like cell xenografts established from paired glioblastoma samples harvested before and after molecularly targeted therapy
Intratumoural heterogeneity underlies tumour escape from molecularly targeted therapy in glioblastoma. A cell-based model preserving the evolving molecular profiles of a tumour during treatment is key to under...
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Article
Single agent efficacy of the VEGFR kinase inhibitor axitinib in preclinical models of glioblastoma
Anti-angiogenic therapy is a promising therapeutic strategy for the highly vascular and malignant brain tumor, glioblastoma (GBM), although current clinical trials have failed to demonstrate an extension in ov...
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Article
Open AccessTreatment of human hepatocellular carcinoma by the oncolytic herpes simplex virus G47delta
Oncolytic herpes simplex virus (HSV) can replicate in and kill cancer cells while sparing the adjacent normal tissue. Hepatocellular carcinoma (HCC) is amongst the most common and lethal cancers, especially in...
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Article
Directed evolution of adeno-associated virus for glioma cell transduction
Glioblastoma multiforme (GBM) is a serious form of brain cancer for which there is currently no effective treatment. Alternative strategies such as adeno-associated virus (AAV) vector mediated-genetic modifica...
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Article
Open AccessFlip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases
Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated ...
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Chapter
Development of Oncolytic Replication-Competent Herpes Simplex Virus Vectors
Oncolytic virus therapy is a promising new strategy for treating cancer that involves replication-competent virus vectors that can replicate in situ in tumor cells, exhibit oncolytic activity by direct cytocidal ...
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Article
Analgesia and hyperalgesia from GABA-mediated modulation of the cerebral cortex
It is known that pain perception can be altered by mood, attention and cognition, or by direct stimulation of the cerebral cortex1, but we know little of the neural mechanisms underlying the cortical modulation o...
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Article
Oncolytic herpes simplex virus vectors for cancer virotherapy
Oncolytic herpes simplex virus type 1 (HSV-1) vectors are emerging as an effective and powerful therapeutic approach for cancer. Replication-competent HSV-1 vectors with mutations in genes that affect viral re...
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Article
Evaluation of ganciclovir-mediated enhancement of the antitumoral effect in oncolytic, multimutated herpes simplex virus type 1 (G207) therapy of brain tumors
G207 is a multimutated, conditionally replicating herpes simplex virus type 1 (HSV-1) that retains an intact viral thymidine kinase (HSV-tk) gene. The virus exhibits oncolytic activity in various tumors and is...
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Chapter and Conference Paper
Experimental Therapy for Malignant Brain Tumors Using Genetically Engineered Herpes Simplex Virus Type 1
We are exploring a novel experimental treatment for malignant brain tumors utilizing a genetically engineered, attenuated, replication-competent herpes simplex virus type 1 (HSV-1). Our previous studies demons...
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Article
Attenuated multi–mutated herpes simplex virus–1 for the treatment of malignant gliomas
We have created a double mutant of the herpes simplex virus (HSV) type 1 (termed G207) with favourable properties for treating human malignant brain tumours: replication–competence in glioblastoma cells (and o...
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Article
Inhibition of angiogenesis and growth of human non-malignant and malignant meningiomas by TNP-470
Meningiomas are relatively common (22%) vascular brain tumors. 3–11% of meningiomas are malignant, and defy currently available therapy. Inhibition of neovascularization is one potential strategy for treating ...
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Article
Mutant herpes simplex virus induced regression of tumors growing in immunocompetent rats
Herpes simplex virus (HSV) mutants kill dividing tumor cells but spare non-proliferating, healthy brain tissue and may be useful in develo** new treatment strategies for malignant brain tumors. Two HSV mutan...
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Chapter
In Vitro Replication of Mutagen-Damaged DNA: Sites of Termination
We have examined the effect of DNA lesions, which in vivo are potentially mutagenic, on in vitro DNA synthesis carried out by a number of purified DNA polymerases using a ØX174 template. Both acetyl aminofluor...
