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Article
Cultivar dependence of transformation rates in moth bean after co-cultivation of protoplasts with Agrobacterium tumefaciens
A simplified protoplast regeneration system for Vigna aconitifolia was developed. A plating efficiency of 60% was obtained using mesophyll protoplasts from 10-day-old seedlings. By co-cultivation of protoplasts w...
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Article
Stable transformation of moth bean Vigna aconitifolia via direct gene transfer
Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts trea...
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Article
Callus induction from protoplasts of V. unguiculata, V. sublobata and V. mungo
Protoplasts were isolated from hypocotyl of V. mungo (L.) Hepper or hypocotyl-derived callus of V. sublobata (Phaseolus sublobata Roxb.) and V. unguiculata (L.) Walp (syn. V. sinensis (L.) Saviex Hassk) using an ...
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Article
Regeneration of plants from cultured root explants of mothbean (Vigna aconitifolia L. Jacq. Marechal)
Decapitated seedling root explants of seven cultivars of mothbean (Vigna aconitifolia) cultured on Murashige and Skoog's basal medium without any phytohormone gave rise to plantlets at the end of 4–5 weeks. Addit...
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Article
Two NAD-dependent alcohol dehydrogenases (E.C. 1.1.1.1) in callus cultures of wheat, rye and triticale
Two NAD-dependent alcohol dehydrogenases ADH-1 and ADH-2, under independent genetic control of genes designated as Adh-1 and Adh-2 located on chromosomes 4A, 4B and 4D, have been reported in aestivum wheat (Hart ...