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Article
A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis
Rhomboid family members are widely conserved and found in all three kingdoms of life. They are serine proteases and serve important regulatory functions. In the present study, a novel gene highly expressed in ...
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Article
Subunit Composition and Function of GABAA Receptors of Rat Spermatozoa
GABA triggers mammalian sperm acrosome reaction (AR). Here, evidence is presented, showing that rat spermatozoa contain GABAA receptors, composed of α5, β1 and β3 subunits. The effects of GABAA receptor agonist a...
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Article
Identification of cell types in sertoli cell-enriched cultures by immunocytochemistry and DNA-specific fluorochrome Hoechst 33342
The cell types in Sertoli cell-enriched cultures can be identified by using the DNA-specific fluorochrome Hoechst 33342 staining. This simple, rapid and reproducible procedure can be used with fixed and living...
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Article
A factor potentiating serotonin in the induction of germinal vesicle breakdown in surf clam oocytes
Simultaneous addition of an aliquot of body fluid obtained from the surf clam,Spisula solidissina, enhanced oocyte germinal vesicle breakdown induced with serotonin but not with KCl. When the body fluid and serot...
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Article
Inducement of blood vessel formation by ovarian extracts from mice injected with gonadotropins
Ovarian extracts prepared from immature mice injected with human chorionic gonadotropin (hCG) and pregnant mare serum gonadotropin (PMSG) were assayed for angiogenic activity. The assay method consisted of imp...
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Article
Properties of Ca2+, Mg2+-dependent endonuclease from sea urchin eggs ofArbacia punctulata
Eine Endodesoxyribonuclease wurde aus den Eiern des SeeigelsArbacia punctulata isoliert. Das Enzym braucht sowohl Ca2+ als auch Mg2+ um maximale Aktivität mitTris-HCl bei pH 8. 5 zu erreichen. Die Matrizenaktivit...
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Article
Stimulation of Mg2+-dependent endonuclease activity of rat testis nuclei on incubation with NAD+ in vitro
Inkubation von isolierten Rattenhodenzellkernen mit Nikotinamidadenindinukleotid induzierte die Bildung von Polyadenosindiphosphatribose, eine Hemmung der Ca2+, Mg2+-abhängigen, alkalischen Endonuklease und parad...
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Article
Routes of administration and uptake of tritiated nucleotides
The uptake, incorporation and distribution of tritiated thymidine and uridine into visceral organs of immature female and male rats was studied. Radioautographs of various organs were prepared 5, 15 and 30 min...
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Article
Ovarian hemorrhages induced in immature mice with human placental gonadotropin
Gonadotropin, durch Fällung mit Aceton und Ammoniumsulfat aus menschlicher Placenta (HPG) isoliert und durch Chromatographie an CM- und FEA-Cellulose sowie durch Gelfiltration an Sephadex G-100 gereinigt, erga...
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Article
Stimulation of RNA polymerase activity by histones, polyamino acids, and polypeptide hormones
Decalysin, Polyarginin, Polylysin, Polyornithin, Kalbsthymushistone und Polypeptidhormone stimulieren in niedriger Konzentration die DNA-abhängige RNS-Polymerase-Aktivität vonEscherichia coli, während sie in hohe...
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Article
Interconversion of cortisol and cortisone in theMacaca mulatta
Die Umwandlung von Kortisol in Kortison und umgekehrt wird nach Injektion von radioaktiven Steroiden bei einem weiblichen Affen (Macaca mulatta) untersucht. Es konnte festgestellt werden, dass im Blut Kortisol re...
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Article
Effect of actinomycin D on the induction of uterine alkaline phosphatase of growing rats by 17β-estradiol
Es wurde Actinomycin D s.c. an junge Ratten verabreicht. Beobachtet wurde die Wirkung der Substanz auf Körpergewicht, Uterusgewicht sowie auf die alkalische Phosphataseaktivität des Uterus vor und nach 17β-Oestra...