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Article
A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis
Rhomboid family members are widely conserved and found in all three kingdoms of life. They are serine proteases and serve important regulatory functions. In the present study, a novel gene highly expressed in ...
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Article
Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-organizing centre
hSMP-1 is a human sperm membrane protein expressed during development. It is a testis-specific component produced during male germ cell differentiation. Proteins that interact with hSMP-1 were identified by th...
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Article
A novel gene, RSD-3/HSD-3.1, encodes a meiotic-related protein expressed in rat and human testis
The expression of stage-specific genes during spermatogenesis was determined by isolating two segments of rat seminiferous tubule at different stages of the germinal epithelium cycle delineated by transillumin...
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Article
Subunit Composition and Function of GABAA Receptors of Rat Spermatozoa
GABA triggers mammalian sperm acrosome reaction (AR). Here, evidence is presented, showing that rat spermatozoa contain GABAA receptors, composed of α5, β1 and β3 subunits. The effects of GABAA receptor agonist a...
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Article
Identification and expression of epidermal growth factor gene in mouse testis
Epidermal growth factor (EGF) is produced primarily by Leydig cells of human testis. Expression of the EGF gene was assessed in mouse testis during the course of sexual maturation by the application of the RT-...
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Article
Expression of cDNA fragment encoding sperm membrane peptide inE. coli
A secretory high-level expression cloning vector designated as pSBC-20 was constructed by inserting a DNA fragment encoding the signal peptide of ompA protein into pBV 220 vector. Any foreign DNA fragment can ...
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Chapter
An Immunoreactive Human Sperm Antigen in Rat Spermatogenic Cells
Antisperm antibodies have been demonstrated in sera and cervical fluids of infertile subjects (3,5,7,10). The condition is complicated by the findings that there is a marked variability in the antibody titers ...
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Article
Identification of cell types in sertoli cell-enriched cultures by immunocytochemistry and DNA-specific fluorochrome Hoechst 33342
The cell types in Sertoli cell-enriched cultures can be identified by using the DNA-specific fluorochrome Hoechst 33342 staining. This simple, rapid and reproducible procedure can be used with fixed and living...
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Article
A factor potentiating serotonin in the induction of germinal vesicle breakdown in surf clam oocytes
Simultaneous addition of an aliquot of body fluid obtained from the surf clam,Spisula solidissina, enhanced oocyte germinal vesicle breakdown induced with serotonin but not with KCl. When the body fluid and serot...
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Chapter
Characterization of an Antisperm Monoclonal Antibody Inducing Human Sperm Agglutination
Sera and cervical fluids of some infertile subjects of undetermined etiology contain antisperm antibodies inducing sperm agglutination and/or immobilization. These antibodies might be the underlying basis for ...
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Article
Inducement of blood vessel formation by ovarian extracts from mice injected with gonadotropins
Ovarian extracts prepared from immature mice injected with human chorionic gonadotropin (hCG) and pregnant mare serum gonadotropin (PMSG) were assayed for angiogenic activity. The assay method consisted of imp...
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Chapter
Isolation and Characterization of an “hCG”-like Protein from Bacteria
Human choriogonadotropin (hCG) is a glycoprotein hormone normally produced by the trophoblastic tissue of the placenta. However, it has also been reported that nontrophoblastic malignant tumors (Rabson et al.,...
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Chapter
Gonadotropin Produced by a Microorganism
Production of a factor resembling chorionic gonadotropin (CG) by a microorganism named Progenitor cryptocides was reported by Livingston and Livingston (1974) and Cohen and Strampp (1976). The factor was tentativ...
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Chapter
A Bacterial Factor Cross-Reacting with Anti-CG
In the present communication, data will be presented to show that a strain of Streptococcus faecalis produces a factor having immunoreactivity to antiserum raised against the β-subunit of human chorionic gonadotr...
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Chapter
Large Molecular Species of Human Chorionic Gonadotropin
Human chorionic gonadotropin (hCG) is a glycoprotein composed of two non-identical glycosylated subunits, designated as α and β. The α-subunit of hCG is virtually identical to pituitary glycoprotein hormones -...
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Article
Properties of Ca2+, Mg2+-dependent endonuclease from sea urchin eggs ofArbacia punctulata
Eine Endodesoxyribonuclease wurde aus den Eiern des SeeigelsArbacia punctulata isoliert. Das Enzym braucht sowohl Ca2+ als auch Mg2+ um maximale Aktivität mitTris-HCl bei pH 8. 5 zu erreichen. Die Matrizenaktivit...
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Article
Stimulation of Mg2+-dependent endonuclease activity of rat testis nuclei on incubation with NAD+ in vitro
Inkubation von isolierten Rattenhodenzellkernen mit Nikotinamidadenindinukleotid induzierte die Bildung von Polyadenosindiphosphatribose, eine Hemmung der Ca2+, Mg2+-abhängigen, alkalischen Endonuklease und parad...
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Article
Routes of administration and uptake of tritiated nucleotides
The uptake, incorporation and distribution of tritiated thymidine and uridine into visceral organs of immature female and male rats was studied. Radioautographs of various organs were prepared 5, 15 and 30 min...
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Article
Ovarian hemorrhages induced in immature mice with human placental gonadotropin
Gonadotropin, durch Fällung mit Aceton und Ammoniumsulfat aus menschlicher Placenta (HPG) isoliert und durch Chromatographie an CM- und FEA-Cellulose sowie durch Gelfiltration an Sephadex G-100 gereinigt, erga...
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Article
Stimulation of RNA polymerase activity by histones, polyamino acids, and polypeptide hormones
Decalysin, Polyarginin, Polylysin, Polyornithin, Kalbsthymushistone und Polypeptidhormone stimulieren in niedriger Konzentration die DNA-abhängige RNS-Polymerase-Aktivität vonEscherichia coli, während sie in hohe...
