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Article
Development of SNP markers linked to purple blotch resistance for marker-assisted selection in onion (Allium cepa L.) breeding
Purple blotch (PB), caused by Alternaria porri (Ellis) Cifferi, is one of the most destructive diseases of onion worldwide. Rapid development and deployment of resistant onion varieties is the most effective appr...
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Targeted Gene Replacement in Plants Using CRISPR-Cas Technology
The CRISPR-Cas based genome editing systems have been widely used in plant functional genomics research and crop improvement due to its simplicity, high efficiency and specificity. For example, the CRISPR-Cas9...
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MicroRNA-Mediated Insect Resistance in Field Crops
MicroRNA (miRNA)-mediated regulation of cell growth and development, gene translational repression, and stress responses have been extensively evaluated over the last two decades. Recent evidences indicate tha...
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Expanding the Scope of Base Editing in Crops Using Cas9 Variants
Genome editing technologies hold tremendous possibilities towards crop improvement and food security for the future. Among others, base editors have developed as novel editing platforms that facilitates specif...
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Genome Editing Is Revolutionizing Crop Improvement
The ever-increasing human population together with emerging environmental cues from climate change demand for novel innovations in plant breeding and agriculture. Targeted genome editing technologies especiall...
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Genome Editing Toward Rice Improvement
Rice (Oryza sativa L.) serves as the major food source feeding half of the world’s population. In the past century, the pedigree-based conventional, radiation-based mutational and DNA marker-based molecular breed...
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Article
A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.)
T-DNA-free homozygous mutant lines developed through a single transcript CRISPR/Cas9 system harboring the desired modification in the CaERF28 locus exhibited significantly enhanced resistance to the anthracnose p...
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Article
Genome wide identification and expression analysis of pepper C2H2 zinc finger transcription factors in response to anthracnose pathogen Colletotrichum truncatum
Although, the C2H2 zinc finger (ZF) family of plant transcription factors have been implicated in multiple biological processes, they are yet to be characterized in the economically important chilli pepper (Capsi...
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Article
Engineering drought tolerance in plants through CRISPR/Cas genome editing
Drought stress is primarily responsible for heavy yield losses and productivity in major crops and possesses the greatest threat to the global food security. While conventional and molecular breeding approache...
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Article
Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
Anthracnose, caused by Colletotrichum spp. is the most devastating disease of chili (Capsicum annuum) in the tropical and subtropical regions of the world. The present study aimed at molecular map** and develop...
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Genome editing technologies and their applications in crop improvement
Crop improvement is very essential to meet the increasing global food demands and enhance food nutrition. Conventional crop-breeding methods have certain limitations such as taking lot of time and resources, a...